Gram Positive Bacilli (MML) Flashcards
False about Corynebacterium diptheriae
A.contains polyphosphate granules
B.granules produces a metachromatic stain in sheep blood agar
C. Brown to black colonies on tinsdale agar which contains sodium tungstenite
D. B and C
E. A, B and C
D. B & C
Correct:” polyphosphate granules which produces a metachromatic stain in Loeffler’s agar “ & “Brown to black colonies on tinsdale agar which contains potassium tellurite “
False about Corynebacterium A. Anaerobic & rod-shaped B. Pleomorphic C. Does not form spores D. None of the above
A. Anaerobic & rod-shaped
Aerobic dapat
Which fragment of diptheria toxin inhibits protein synthesis & is metabolically active?
A. Fragment A
B. Fragment B
C. Both fragments
A. Fragment A
True or false: fragment A is required for transport of fragment B
Baliktad, so bale false
[DIPTHERIA TOXIN]
Sequence the ff
1.A and B unfolds
2.A goes freely across cytoplasm to attack elongation factor 2 (EF2)
3.B subunit will bind with surface receptors of epith cells
4.Low pH, so toxin will be transferred to cytosol
5.Toxin A attacks another ribosome or cell
6.ADP Ribosylation inactivates EF-2
3,4,1,2,6,5
All are media for diagnosis of cutaneous diptheria except A.Trypcase agar with 5% sheep blood B.Cystine-tellurite C.nutrient agar D.Loeffler's slant E.Modified Tinsdale
C.nutrient agar
Which is false?
A.Blood or serum tellurite agar stimulates the normal flora metabolized by corynebacteria.
B. Cutaneous diptheria is more infectious than respiratory diptheria
C. Both are false
D. Both are true
A is False. Inhibit dapat. Kaya siya diagnostic medium for cutaneous diptheria
Purpose of tellurite in medium
Inhibits normal flora
False about biotypes (C.diptheriae):
a. Gravis - large non hemolytic b.Intermidius - small colonies
c. Bitis- large beta hemolytic
d. C.diptheriae only has three biotypes
d. C.diptheriae only has three biotypes
False, four biotypes! Last is Belfanti.
Manifestation of pharyngitis caused by C.diptheriae
Thick adherent green pseudomembrane
Which is false?
A. C.diptheriae may cause bull neck to be visible in the afflicted patient
B. DPT vaccine for adults is the same as the one for kids
C. May be spread through Droplet/contact of respiratory disease
D. May be spread through cutaneous Discharge from extracutaneous sites
B. DPT vaccine for adults is the same as the one for kids
False about Listeria monocytogenes A. non spore forming rod B. Catalase negative C. Tumbling end-over-end motility at 22-28°C D. Can survive ref temp
B. Catalase negative
Positive dapat
False about Listeria monocytogenes A. Alpha hemolysis B. Grows of Mueller-Hinton agar C. Facultative anaerobe D.Esculin hydrolysis positive
A. Alpha hemolysis
Beta dapat
False about Listeria monocytogenes
A.non-Motile
B.Produces acid but not gas in a variety of carbohydrates
C.Also cause neonasal sepsis and meningitis
D. None of the above
A.non-Motile
How does Listeria monocytogenes enter the body?
Via the GI Tract
How does Listeria monocytogenes initiate phagocytosis?
Surface protein called internalins interact with E-cadherin (receptor on epithelial cells) promoting phagocytosis
After phagocytosis, there ensues a local reorganization of cytoplasm. Where does Listeria monocytogenes enter next?
Vacuole
Once Listeria escapes, it proliferates and Act A, another listerial surface protein, induces ______.
host cell actin polymerization
Once host cell actin polymerization ensues, Listeria then is propelled to the___
Cell membrane
Once Listeria is at the cell membrane, these elongated protrusions are formed & Ingested by adjacent epithelial cells, macrophages and hepatocytes
Filopods
Which is false about Listeria monocytogenes?
A)Usurps host cell’s actin and contractile system to spread infection
B)Listeria produce siderophores and are able to obtain calcium from transferrin
C)both a and b
D)none of the above
B)Listeria produce siderophores and are able to obtain calcium from transferrin
Iron dapat
- Result of infection in the uterus and is disseminated form of disease (still birth)
- characterized by neonatal sepsis, pustular lesions, granulomas containing L. monocytogenes in multiple organs
What is this?
Granulomatosis infantiseptica
False about L.monocytogenes
A. May be acquired through birth canal (3 week delay of infection)
B. Has tropism for CNS
C. May be obtained by immunosuppressed adults,
D. Obtained from stool sample
D. Obtained from stool sample
Nope,from blood or spinal fluid dapat
All can treat Listeriosis except A.Ampicillin B.Cephalosporin C.Erythromycin D.Intravenous trimethoprim-sulfamethoxazole
B.cephalosporin
False about Listeria monocytogenes A. Food borne transmission from animal products is impossible B. Cold growth enhances infectivity C. Both of them D. None of them
A. Food borne transmission from animal products is impossible
False about Bacillus A.aerobic rods B. occurring in chains C. Mostly Saprophytic organisms which are prevalent in soil, water, air, and on vegetation D. Do not Form spores
D. Do not Form spores
False about spores of Bacillus anthracis
A.Formed in culture, in the soil, and in tissues and exudates from dead animals.
B. Formed also in the blood and tissue of living animals
C. Remain viable in soil for decades D.Germinate at the site of entry
B. Formed also in the blood and tissue of living animals
Dead animals only
When Bacillus anthracis infects man via inhalation, what disease is this?
Woolsorter’s disease
Main component of Bacillus anthracis ‘ ,capsule
poly-D-glutamyl
False about Bacillus anthracis
A. Herbivorous animals are safe from this
B. Contaminated meat may be means of infecting man
C.Gene virus is carried by plasmids
D.has edema factor and lethal factor
A. Herbivorous animals are safe from this
In fact, sila yung pinaka-at risk
False about cutaneous anthrax
A. Less common and less deadly than pulmonary anthrax
B. Small papule that becomes vesicular
C.black eschar at the base surrounded by a zone of induration – malignant pustule
D.Spores enter through small breaks in skin
A. Less common and less deadly than pulmonary anthrax
More common dapat
False about pulmonary anthrax
A)spores are eliminated by alveolar macrophages
B)Hilar lymph node with marked hemorrhagic necrosis may occur
C)Death results within 24 hours
D)may be used in biological warfare
A)spores are eliminated by alveolar macrophages
They are still able to germinate and replicate
Gastrointestinal anthrax is found mostly in \_\_\_ animals; A)carnivorous B)herbivorous C)omnivorous D)all
B)herbivorous
All can be used as diagnostic specimen for Bacillus anthracis except A)blood B)fluid C)pus D)stool E)sputum
D)stool
Which is false about B.anthracis?
A. Nonmotile
B. Carb fermentation useful
C. Non-hemolytic gray/white mucoid colonies at BAP
D. Capsule can be microscopically visualized via India ink
B. Carb fermentation useful
Which is false about B.anthracis?
A.infection is through injection of spores from herbivores into the skin
B.doxycycline can be used to treat anthrax
C.antibiotics have minimum effect on cutaneous anthrax
D. Vaccine is commercially available
D. Vaccine is commercially available
False about Bacillus cereus A. Forms spores B.facultative C. Short generation time D. Acid from all sugar fermentations
D. Acid from all sugar fermentations
All sugars Except mannitol
False about B.cereus A. Mildly proteolytic B. When it lyses protein, it produces foul smell C. Carried by humans and animals D.Both a and b
B. When it lyses protein, it produces foul smell
No foul smell, kaya baka naiingest mo na pero hindi mo napapansin
False about emetic toxin of B.cereus A. Stimulates vagus affrebt B. Antigenic C.resistant to heat, pH or proteolysis D. May cause vomiting
B. Antigenic
Hindi siya antigenic.
B.cereus produce ___ colonies on BAP after 24hr at 37 degrees Celsius
Anthracoid
False about diarrheal enterotoxin of B.cereus
A)disrupt membrane of epithelial cells
B)less potent than Clostridium perfringes
C)both a and b
D)none of the above
B)less potent than Clostridium perfringes
More potent dapat
False about Bacillus cereus
A)resistant to polymyxin
B)ferments mannitol
C)colonies appear pink
B)ferments mannitol
Commercial kits for enterotoxin detection include __ and __
A)reverse passive latex agglutination
B)enzyme immunosorbent assay
When L.monocytogenes is engulfed by the phagolysosome, and the pH becomes low, this substance is released, allowing Listeria to escape into the cytoplasm.
Listeriolysin O
