Enteric Viruses Flashcards
Rotavirus is a Reovirus. What does REO mean?
REOVirus : - Respiratory Enteric Orphan Virus
genome of Reovirus
double stranded RNA
False about Reovirus virion
A.Two concentric helical capsid with capsomeres
B. contains several enzymes for transcription
and capping of viral RNA
C. both
A
icosahedral dapat
False about Reovirus
A. no envelope
B. replication is at nucleus & virions not completely coated
C. stable to heat, 3-9pH & lipid solvents
D. inactivated by 95% ethanol, phenol, chlorine
B
cytoplasm dapat
Reovirus is stable in all except A)heat B)95% ethanol C)3-9 pH D)lipid solvents
B
Rotavirus group __ : primary human pathogens
A, B, C, D
A
For Rotavirus: outer capsid proteins used for serotyping
VP4, VP7
protein in intermediate capsid (rota virus)
VP6
protein in inner capsid (rotavirus)
VP2
Mature form is __
A. triple-shelled capsid
B. double-shelled capsid
C. single-shelled capsid
A
Immature form is __
A. triple-shelled capsid
B. double-shelled capsid
C. single-shelled capsid
B
viral protein in Rotavirus for binding & defining P serotype
VP4
viral protein in Rotavirus for defining G serotype
VP7
Rotavirus attaches via VP4 to what host cell receptor?
sialic acid
in rotavirus
what cleaves VP4 to VP8 and VP5?
Trypsin enzyme
reduction in __ of outercapsid leads to removal of VP7. releasing double shelled virus
Ca
What do VP1 and VP3 facilitate? for rotavirus
A. significant parts of central dogma
B. attachment
C. virus assembly
A
transcription, translation, protein syn
Rotavirus lyses the cell. T or F?
T
Most common G serotype all over the world for rotavirus
G1
Most common G serotype in country for rotavirus
G3
viral enterotoxin c/o rotavirus that induces diarrhea
non-structural protein 4
which parts of GIT are most affected by rotavirus?
proximal duodenum to proximal jejunum
which is false?
A. rotavirus only takes small inoculum to develop disease
B. rotavirus affects gastric and colonic mucosa
C. rotavirus may also cause lack of sugar degradation
D. more common during cold months
B
clinical triad of rotavirus
fever, vomiting, diarrhea
rotavirus has respiratory symptoms, t or f?
t
REOvirus!! symptoms: cough, coryza
most sensitive method of diagnosis for rotavirus
PCR
Rotavirus can be cultured in media with __
trypsin
vaccine for rotavirus is
A.combination
B.pure polysaccharide
C.live attenuated
C
given orally, too :)
official name of norovirus
norwalk-like viruses
family of norovirus
Caliciviridae
false about norovirus A. 6 =number of genogroups @ norovirus B. genome is (+)ssRNA C. virion is icosahedral D. enveloped virus
D
naked say
defining char of norovirus surface
cup like depression
non-structural protein from RNA involved in RNA transcription
VPg
norovirus affects what part of GIT?
Proximal duodenum
proximal jejunum
enumerate what is/are malabsorbed due to norovirus
fat, lactose and D-xylose
most common clinical manifestation of norovirus
stomach flu
Once you have been infected with Norovirus, you are forever immune. true or false?
false;no lifelong immunity
most widely used technique to detect Caliciviruses
PCR
Vaccine is available for people who wish to not get Norovirus. T or F?
F
Genus of Astrovirus
Mamastovirus
false about Astrovirus A. icosahedral virion B. single strand negative sense RNA C. no envelope D. stable in acid and resistant to heat
B
positive!!
most important capsid proteins of astrovirus
VP26 and VP29
symptomatic duration for astrovirus
3-4 days
most useful way of diagnosing astrovirus
electron microscopy
A. orofecal transmission
B. highest infection rates in summer months
C. low infective dose
D. symptomatic treatment
False about astrovirus
B
rainy dapat
body’s mechanism of immunity vs rotavirus
local intestinal IgA
