FOM Week 1 Flashcards
What is the purpose of the cytoskeleton?
Structural strength, transport, movement and directional info
What does it mean when a cytoskeleton is dynamic?
location and life cycle can alter the protein output and ratios
At what end does actin tend to have subunits added to?
The plus end, but can have units added to the minus end
What important molecule does actin have a binding site for?
ATP binds right in the center
What are ARPs? What do they do?
ARPs for a complex and bind to the minus end of the actin filament, stabilizing it. Filament will then grow rapidly on plus end. They also bind other acting molecules at a 70 degree angle and create a meshwork
How do microtubules compare to actin?
They are thicker and consist of heterodimers of alpha and beta tubulin subunits 13 protofilaments come together
What are the locations of the plus and minus end of a microtubule?
Plus end lies in the periphery and the minus end is in the centriole
What is the purpose and outcome of the GTP cap on microtubules?
GTP cap stabilizes microtubule allowing for growth
What happens to a microtubule with a GDP cap?
a GDP cap is unstable, the microtubule will begin to shrink GDP encourages curving of the protofilament which can lead to shrinking
What can be used to stabilize the minus end of a microtubule?
Gamma tubulin
What is formin?
Dimer that allows actin to grow faster by stabilizing the plus end and adding subunits
What is profilin?
binds actin to enhance ATP binding and adds actin subunits to the plus end
What is cofilin?
binds actin and condenses it leading to a shorter and thicker filaments
What is a subunit example of a microfilament?
actin
What are a few subunit examples intermediate filaments?
Vimentin (mesenchyme) Glial fibrillary acidic proteins (glia) Neurofilament (neurons) Keratins (epithelia) Nuclear lamin Desmin (muscle)
What is a subunit example of microtubules?
alpha and beta tubulin
What are the common structural properties of fibrillar proteins?
Repeating globular subunits and long twisting fibers Building structures for cytoskeleton or ECM Actin tubulin - repeating alpha and beta subunits creating protofilaments
What are the common structural properties of integral membrane proteins? What are examples?
Surrounded by hydrophobic lipid membrane Inside out distribution of amino acids - hydrophobic amino acids span the membrane and hydrophilic sit outside the membrane Bundles of alpha helicies and beta barrels
What are the common structural properties of intrinsically disordered proteins?
- Not globular 2. Unpredictable secondary structure 3. No hydrophobic core 4. Long repetitive sequences 5. Appear to fold when binding targets 6. Bind wide range of targets
Explain the characteristics of proteins with similar sequences
These are homologs Can have as little as 25% of same seq but have similar function Difference in residues lead to individual characteristics
What are the functional classes that proteins can share?
Chemical Signaling structure transport storage
Provide an example of an isoform and explain what it is.
Hexokinase (phosphorylates many sugars) vs glucokinase (phosphorylates just glucose) Isoforms have same general function but subtle differences
Can proteins share a common ligand? If so explain.
Yes Many proteins can bind ATP and have similar binding sites for this to occur
What are two common post-translational modifications?
Phosphorylation and proteolysis
What is phosphorylation
Protein kinase removes a hydroxyl and the addition of a phosphate group. Can activate or deactivate a protein
What are the characteristics of proteolysis?
Proteases cleave the phosphate backbone Create a new N and C terminus Irreversible
What is a characteristic of protein modularity?
Mix and match of different classes on one polypeptide
What is a domain?
A domain is a part of a polypeptide that may have its own specific function Each domain may have its own characteristics and the function of the whole may not be knocked out by deletion of one domain
What are the types of folding?
On purpose or accidential
What is an example of a protein unfolding on purpose?
Protein digestion in the stomach as well as lysosomal protein degradation
What can happen what a protein is accidentally misfolded?
It can be targeted for degradation
What is cytoplasmic crowding and what can be a result of it?
The cytoplasm is very crowded and proteins can push up against each other possibly leading to protein unfolding. This can expose hydrophilic sidechains that will glomb to other side chains and aggregate together
What factors can cause a protein to denature?
pH, Ionic strength, Pressure, Temp, Urea, Osmotic Pressure
What is a pathological result of protein unfolding?
Amyloid formation can occur when unregulated protein aggregation occurs from different native folding
What factors can enhance folding?
Osmolytes, chaperone proteins, and chaperonins
What is an osmolyte and what are some examples of osmolytes?
Component that affects osmosis Amino acids, polyols (glycerol), methylamines (TMAO), Covalent osmolytes
What is an example of a chaperone protein and what do they do?
Heat shock proteins Aid in folding new proteins, help in misfolded proteins and transport across a membrane These bind to smaller sections and aid in folding
What are the characteristic and functions associated with chaperonin proteins?
Much larget than HSPs barrel structure provides inner cavity for protein folding Cytosolic protein Lid shuts on the proteins Proteins can go through chaperonins multiple until desired folding is achieved
What are the major degredative pathways in a cell?
Lysosomes and Proteosome
What are the characteristics of a lysosome?
Low pH to unfold protein and acid proteases to degrade the proteins
What are the characteristics of a proteosome?
Large cytosolic protein and utilizes ubiquitin tagging
How can degradation play a part in a role such as cystic fibrosis?
Mutation leads to slower protein folding, and thus the protein is degraded before it can fold
What intermediate filament is characteristic of muscle
Desmin
What intermediate filament is characteristic of epithelia?
Keratins
What intermediate filament is characteristic of neurons?
Neurofilaments
What intermediate filament is characteristic of glia?
Glial fibrillary acidic protein
What intermediate filament is characteristic of mesenchyme?
Vimentin
What proteins act to stabilize actin bundles?
Fimbrin - tight, doesnt allow myosin to connect creates parallel bundles Spectrin Actinin - allows for myosin creating contractile bundles Filamin dimers- creates and stabilizes actin meshwork
Why do so few actinopathies exist? Why do the genetic codes of all actin proteins seem to be so closely related?
Actin is extremely important and most embryos with actin mutations rarely make it past the 16 cell stage
What protein can stabilize the minus end of a microtubule?
Gamma tubulin
What two microtubule associated proteins play important roles in neurons?
MAP2 and Tau proteins
Describe the characteristics of a MAP2 protein?
Binds adjacent microtubules and allows for correct spacing of microtubules
What is the tole of Tau proteins?
These bind microtubules and pack them in tighter bundles.