FOM 5.4.3 Flashcards
At what point during gene transcription does it leave the nucleus?
After post-transcriptional modifications are done the mRNA is exported out of the cell
Where is the nucleus and nucleolus in this picture?
You know where they are dipshit
The dark spots of the nucleolus are known as?
The dense fibrillar component
What is the life cycle of snRNPs?
Newly synthesized snRNAs are exported to the cytoplasm where they join to create partially assembled snRNPs. The snRNPs are then brought back into the nucleus, shipped into the nucleolus where they are fully assembled.
What is the process by which ribosomes are created?
rRNA is transcribed using Pol 1 (refer back to Dr. Fontes’ lecture). The rRNA is then processed and modified. The rRNA will then complex with ribosomal proteins. The proteins that modify the rRNA are made in the cytoplasm and shipped into the nucleolus. The proteins that join with the rRNA to make the ribosome are also made in the cytoplasm and shipped into the nucleus (nucleolus). The ribosomal subunits are then processed and exported through the nuclear pore complex. Once out in the cytoplasm, they bind to mRNA and together with tRNA will make proteins.
Take your best guess at what is imported vs exported through the nuclear pore complex.
How come nuclear export proteins arent found in the cytoplasm?
Because they are removed upon exit of the mRNA from the nucleus
What is the process of the protein and RNA shuttling complex in the nucleus?
Proteins that interact with Ran-GDP are brought into the nucleus from the cytoplasm
Trading of GDP for GTP then causes the protein to dissociate
Shuttling out protein associates with Ran GTP - and leaves with the GTP. GTP dissociation then causes the protein dissociate
Is the ER attached to the nucleus?
Yes how have I not been taught this before?!?!?
What are the pink arrows pointing at?
Ribosomes
What is the main way a protein gets inserted into the membrane?
It is referred to as co-translational, occurring while translation is still happening. Look at the picture, there is an ER signal sequence, and remains bound to ER while translation is occurring
How are proteins sent to the golgi?
They are placed in an ER forming transport vesicle. Which is sent there. The key to this is that chaperones are working on making sure correctly folded proteins are in the vesicles, and misfolded proteins are out of the vesicle.
What is this a pic of?
THE GOLGI!!!!!
What is an important characteristic of the golgi moving from cis to trans?
Moving from cis to trans it is important to know that the enzymes within the golgi are changing as you move more trans
Can you name the differenct components of this pic?
Top - Nuclear envelope
Below that - rough ER
Middle long line - vesicular tubular clusters
Bottom - cis Golgi
