FOM 5.5.1

Question 1

What are the steps in which a vesicle can move from a donor compartment to a target compartment?

Answer 1

Budding (energy required), Fusion

Question 2

What are the three flavors of vesicles?

Answer 2

clathrin, COPI, and COPII coated

Question 3

What are the proteins of the vesicle coat used for?

Answer 3

sorting, routing, and fusion

Question 4

What process is clathrin associated with?

Answer 4

Endocytosis (budding of the PM compartment

Question 5

What protein binds the receptor, which then links to clathrin?

Answer 5

AP-2 (adaptor protein 2)

Question 6

Describe the process of forming a clathrin coated vesicle

Answer 6

1) Receptor bound by AP-2
2) Aggregation of AP-2 bound receptors
3) binding of clathrin/formation of an envagination
4) Endocytosis of vesicle
5) Dissociation of AP-2 and clathrin

Question 7

What small GTPase of the Rab family recruits COPII?

Answer 7

Sar1

Question 8

Which GEF causes the GDP/GTP conversion of Sar1 that causes the insertion of Sar1 onto the ER?

Answer 8

Sec12

Question 9

Once Sar1 is phosphorylated and inserted into the membrane, what protein is recruited?

Answer 9

COPII