Enzymes Flashcards
Cellular energy
Stored in the bonds of biomolecules
What are enzymes
Catalysts made from proteins
What are enzymes made from
Chains of amino acids (folded into 3D)
What will effect the efficiency of an enzyme
Ph
Temp
What are catabolic enzymes
Breaks down substrate into simpler parts
Amylase
Example of an anabolic enzyme
DNA ligase
Why is temperate important for enzyme activity
If it is too cold cell contents freeze
If it is too hot molecular movement increases and loose 3D shape(denatured)
Methods of immobilising enzymes
Attqtch to eachother
Attach to insoluble supports
Enclosed within a membrane or gel
Advantages of immobilising enzymes
Can be reused
Can remain in the vessel at the end of the process
Increases stability
Cheaper
Issues of immobilised enzymes
Soft drinks sweetened with fructose
Lactase used into milk
Experiment acid
Hydrogen peroxide
Preparing enzyme immobilisation
Sodium alginate, yeast, calcium chloride
What is bioprocessing
The use of controlled enzyme reactions to produce a product
What is a bioreactor
A vessel or container in which living cells or their products are used to make a product
