diagnostics

Question 1

What is the animal immunized with to create antibodies

Answer 1

innactivated pathogens

Question 2

What are monoclonal antibodies used to detect

Answer 2

Pathogens

Antibodies

Question 3

What are monoclonal antibodies made from

Answer 3

mouse antibody produced by a single clone of b cells

specificity for a single epitope

Question 4

Direct immunofluorescent antibody test

Answer 4

detection o f pathogen-specific antigens in tissues or blood smears
Diagnosis of infections
Specific for pathogens

Question 5

What is a disease that direct immunofluorescent antibody test is used for

Answer 5

FeLV

Rabies

Question 6

Indirect immunofluorescent antibody test

Answer 6

Detection of pathogen-specific antibody
Usually serum
Patient’s IgG

Question 7

Difference between indirect and direct immunofluorescent antibody test

Answer 7

Direct- Specific antigen

Indirect- Specific antibody

Question 8

What is a disease indirect immunofluorescent antibody test can be used to detect

Answer 8

ehrlichia canis from ticks

Question 9

What is ELISA

Answer 9

enzyme-linked immunosorbent assay

Question 10

what does ELISA test for

Answer 10

test for antigens or antibodies in patient samples

Question 11

Antigen capture assay

Answer 11

Type of ELISA

Detect pathogens

Question 12

Antibody capture assay

Answer 12

Type of ELISA used to Detect antibodies

Question 13

What is in antigen capture ELISA well

Answer 13

Capture Ab to Agent X
Patient serum with agent X
HRP-detection Ab for agent X
HRP substrate

Question 14

What’s in an anitbody capture ELISA well

Answer 14

Capture Ag
Patient serum with Ab to agent X
HRP-detection Ab
HRP substrate

Question 15

What’s one problem with antibody ELISA

Answer 15

can’t differentiate between infected and immunized

Question 16

Polyclonal antibodies

Answer 16

Raised in an animal to detect pathogens

Question 17

Hemagglutination

Answer 17

cross linking of RBC’s

Question 18

What are 2 hemagglutination assays

Answer 18

Coombs test

Blood typing

Question 19

What does Coombs test detect

Answer 19

antibodies to RBC

Question 20

What does hemagglutination assays detect

Answer 20

RBC antigens

determine blood type

Question 21

What does hemagglutination inhibition assay measure

Answer 21

Antibodies to viruses that naturally agglutinate RBC’s

Question 22

What test do you use for canine parvovirus

Answer 22

Hemagglutination inhibition assay

Question 23

What is serum neutralization assay used for

Answer 23

detection of virus specific antibodies

Question 24

What types of viruses are serum neutralization assays used for

Answer 24

viruses that don’t agglutinate RBC

Question 25

What are antibody titer assays used for

Answer 25

estimate the amount of pathogen-specific Ab in serum

Question 26

Antibody titer

Answer 26

reciprocal of the highest serum dilution that gives a positive result in the test