Antigen processing and presenting

Question 1

Where is MHC 2 found

Answer 1

Only on APC cells

Question 2

What are the three professional APC

Answer 2

Dendritic cell
B Cell
Macrophage

Question 3

What are some characteristics of dendritic cells

Answer 3

Best APC
only one that can stimulate naive cell
Regulate both innate and acquired immune systems
Can take up many different antigens

Question 4

Where do dendritic cells come from

Answer 4

bone marrow

Question 5

What makes an immature DC into a mature DC

Answer 5

antigen

Question 6

What is proteolysis

Answer 6

protein degradation

Question 7

What is phagolysosome

Answer 7

digestive enzymes in the lysosome

Question 8

Where are antigens presented

Answer 8

MHC-peptide complex on cell surface

Question 9

How do we uptake endogenous antigen

Answer 9

Sample of intracellular proteins

Viruses, intracellular bacteria

Question 10

How does degradation of endogenous antigens happen

Answer 10

Ubiquitin-chaperone protein, brings protein to the proteosome
Proteosome- group of enzymes that digest proteins

Question 11

What is the complex formation of endogenous antigens

Answer 11

MHC class 1

Question 12

What is the presentation of endogenous antigens

Answer 12

MHC class 1 on all nucleated cells

Question 13

What are processed and presented on MHC class 1

Answer 13

all intraceytoplasmic proteins

Question 14

What recognizes foregin MHC 1

Answer 14

CD8 cytotoxic T cells

Question 15

What do NK cells recognize on cell and what happens

Answer 15

Lack of MHC 1, if there is none it is killed by NK

Question 16

What causes the uptake of exogenous antigen

Answer 16

Phagocytosis, endocytosis, BCR-mediated

Question 17

What does MHC 2 present on

Answer 17

only on antigen presenting cells

Question 18

What does MHC class 2 cells do

Answer 18

presents a sampling of the proteins outside of the cell

Question 19

What recognizeses MHC class 2 cells

Answer 19

CD4 helper T cells

Question 20

What does MHC class 2 activate

Answer 20

acquired immune system

Question 21

Is MHC 2 stable without an antigen

Answer 21

No

Question 22

What recognizes MHC class 1

Answer 22

CD8

Question 23

Is MHC stable without antigen

Answer 23

no

Question 24

Endogenous vs Exogenous

Answer 24

Endogenous: All nuc. cells, intracellular antigen, virus, proteozome, Ag loading on ER, MHC 1, CD8 T-cell

Exogenous: Only on APC, extracellular antigen, bacteria, fungus protozoa, phagolysosome, Ag loading in phagolysosome, MHC 2, CD4 T-cell

Question 25

What is the “rule of 8”

Answer 25

MHC 1 goes with CD8 T-cell
MHC 2 goes with CD4 T-cell

1x8=8
2x4=8

Question 26

What do CD4 T cells do if they make IL-2, IFN-gamma

Answer 26

If they do this they are Th1

They just help, don’t do any of the work

Question 27

What does a Th1 response do

Answer 27

Stimulate cell mediated killing

CD8 cytotoxic T cell does the killing

Question 28

What does CD4 T cells that make IL-4, IL-5 do

Answer 28

Th2

Stimulate humoral response (B-cells)

Question 29

What do effector cells do

Answer 29

Surveying for infected cells and killing or producing antibody

Question 30

Characteristics of CD8 T cells (cytotoxic effector cells)

Answer 30

Intracellular antigens

Recognize MHC 1

Question 31

What causes the exogenous degredation

Answer 31

phagolysosome

Question 32

What is the complex formation of exogenous antigen

Answer 32

MHC 2

Loaded in phagolysosome

Question 33

How is exogenous antigen presented

Answer 33

MHC 2 only on APC

Question 34

How do APC’s function by

Answer 34

Internalizing antigen
Digesting antigen
Display peptide Ag bound to MHC 2