Design features chromatography Flashcards
1
Q
Grind up leaves with sand
A
To rupture cells and release pigments
2
Q
Use acetone
A
To dissolve leaf pigments
3
Q
Filter mixture
A
To separate solution of pigments from cell debris
4
Q
Nettle or lettuce leaves used
A
Wide range of pigments present
5
Q
Dry between application of extract
A
To keep spot of extract small
6
Q
Repeated spotting of pigment
A
Intense spot obtained
7
Q
Reduce the extract by evaporation
A
Increases the concentration of pigments in the extract
8
Q
Measuring cylinder wrapped in foil
A
Prevents fading of pigments
9
Q
Keep pigment spot above solvent level
A
Prevents pigments bleeding into solvent
10
Q
Remove chromatogram before solvent reaches the top of paper
A
Prevents the separated pigments from merging
11
Q
Use acetone/petroleum ether solvent
A
To produce clear separation of pigments
