CPRS 43: GBM

Question 1

Name 3 locations with Glycocalyx
1.
2.
3.

Answer 1

Name 3 locations with Glycocalyx

1. Endothelial Glycocalyx
2. Between foot processes and GBM
3. On the surface of podocytes

Question 2

GBM is negative, it will repel large negative molecules like albumin. Therefore, the Bowman’s capsule right after ultrafiltration is less negative.

We need to compensate the lowered negative charge by _________________

Answer 2

GBM is negative, it will repel large negative molecules like albumin. Therefore, the Bowman’s capsule right after ultrafiltration is less negative.

We need to compensate the lowered negative charge by introducing more negative ions

Question 3

Name the two components of Slit Diaphragm
1.
2.

Answer 3

Name the two components of Slit Diaphragm

1. Nephrin
2. Podocin

Question 4

Name 2 components of the GBM
1.
2.
Also name the type of collagen

Answer 4

Name 2 components of the GBM

1. Proteoglycans
2. Glycoproteins, which contains type IV collagens

Question 5

There is amino terminal and carboxy terminal of a protomer. (refer to diagram on CPRS 43)

Thin end = Amino terminal
Thick end = Carboxy Terminal

Through C terminal dimerization, they form _______.

Through N terminal tetramer formation, they form __________.

Answer 5

There is amino terminal and carboxy terminal of a protomer. (refer to diagram on CPRS 43)

Thin end = Amino terminal
Thick end = Carboxy Terminal

Through C terminal dimerization, they form dimer.

Through N terminal tetramer formation, they form N-terminal tetramer.

Question 6

What substance has

A, B1, B2 chains and a free end that forms a 4-armed structure

Answer 6

Laminin

Question 7

Consider the component Proteoglycan

Name two components

• A
• P

Name 2 associated chains

• H
• C

Answer 7

Consider the component Proteoglycan

Name two components

• Agrin
• Perlecan

Name 2 associated chains

• Heparan Sulfate Chain
• Chondroitin Sulfate Chain

Question 8

Consider the graph of F/P ratio of the substance

What is F/P ratio: amount of the substance in Glomerular filtrate/ amount of the substance in plasma

A larger ratio means a _______ proportion of the substance is filtered in the Bowman’s Capsule. (Smaller or larger)

A higher molecular size will ____ rate of filtration
Increase in negative charge will _____ rate of filtration

What happens during nephritis?

Answer 8

Consider the graph of F/P ratio of the substance

What is F/P ratio: amount of the substance in Glomerular filtrate/ amount of the substance in plasma

A larger ratio means a larger proportion of the substance is filtered in the Bowman’s Capsule. (Smaller or larger)

A higher molecular size will decrease rate of filtration
Increase in negative charge will decrease rate of filtration

What happens during nephritis?
Loss of charge selectivity