3.2 Electron Microscopes Flashcards
What are the pros of Electron microscopes?
Higher Magnification
Higher resolution
Visible sub cellular structures
What are the cons of electron microscopes?
Expensive
Only used on dead cells
What are the pros of light microscopes?
Cheaper
Can be used on living cells
Less colour distortion
What are the cons of light microscopes?
Low magnification
Lower definition
Cannot see sub cellular structure
What are the 2 types of electron microscope?
SEM
TEM
What are the cons of a TEM
Living specimens cannot be observed
It has a complex staining problem
Only in 2d
What are the pros of a TEM
Higher resolution
Allows view of inside the cell
What are the cons of an SEM
Bad resolution
What are the pros of a SEM
Provides a 3d image
How do electron microscopes work?
By firing electrons which bounce if the surface of structures
How do electron microscopes work?
By firing electrons which bounce if the surface of structures