2.2 DNA Replication Flashcards
Why do you need to replicate DNA?
For mitosis
What is the dispersive DNA replication?
Nucleotides break into small bits and new nucleotides fill in the gaps
What is conservative DNA replication?
2 new strands make up a whole new double helix and parent helix is untouched
What is the semi conservative DNA replication?
Each parent strand joins one daughter strand
What was the test to find out which DNA replication was right?
Grow microbes in N15 (Nitrogen 15 isotope) then grow them in N14 then spin in centrifuge, A thick line of N14 sat on top whilst a thin line of N15 sat below his showed it semi conservative and conservitive
What are the requirements for making DNA?
Helicase
Polymerase
DNA template
ATP
Nucleotides
Ligase
What does dna helicase do?
Break hydrogen bonds between nucleotides
What does dna polymerase do?
Copy nucleotides
What does dna ligase do?
Put nucleotides back together