Week 7- Advanced Breeding Techniques Flashcards
What hormone is used to naturally induce ovulation in animals?
estrogen
hormone of estrus
What day would you do foetal sexing?
Around day 60
What would you identify on foetal sexing?
Genital tubercles
Where are genital tubercles seen on ultrasound?
half way betweem umbilicus and anus
What are the industry drivers for repro tech?
- Improved repro efficeicny- increased genetics, no compromise of donor, decreased disease/ injury
- Fertility treatments- sub-optimal semen use, increased fecundity
What is female fecundity used?
when donor animals cannot support a pregnancy or desired no of pregnancies
When is MOET most commonly used?
Dairy cattle- but has a variable response
third- no transferrable embryos
third give 1-2
third have optimal 5- 12
How do you get superovulation?
- Decreasing doses of FSh twice a day
- Superovulation= 36-48 hours after PG injection
- Double AI 12 and 24 hours after heat
- Flushing done 7 days after heat
What are three ways you could collect embryos in a ewe?
- Laparoctomy
- Oviductal flush (1 way)
When should you collect embryos in equines?
Day 6/7 post AI
What are the benefits of collecting embryos from equines?
- Freedom from gestational restrictions
- Non-surgical
- Over 50% pregnancy rate
How would you transfer embryos in bovines?
- Epidural anaesthesia
- Identify the corpus luteum
- Trans-Cervical
- pregnancy rates are 60-70%
What are the two steps to equine ovum pick up?
- transfer to recipient mare
- intracytoplasmic sperm injection
What are the advantages of equine ovum surgery?
- Simple tech
- limited oocyte handling
What are the disadvantages of ovum handling?
- Fresh transfer
- may need surgical intervention
What is the issue with bovine IVF?
- Dont freeze well
- Higher rate of pregnancy loss
small embryo development rate
What are the positives of bovine IVF?
- can be performed at any stage
- large quantities of oocytes collected
- reduced semen costs
