Unit 1.1b Flashcards
How can accuracy be checked?
By using properly calibrated equipment or checking measurements with a different system
What does accurate mean?
Closeness of a measured value to the true value.
What does precise mean?
The closeness of repeated measurements
What does reliable mean?
The overall consistency of a measure, and if same results can be achieved every time
How do you make an experiment reliable?
By repeating it
When would you use a linear dilution series?
If you need a sample over a reasonably small range
How do you make a linear dilution series?
Volume from stock solution is diluted using a solvent to bring
Does a mistake in measurement for linear dilutions have an affect on all?
No, it will only impact one concentration
What is a log dilution?
Dilution that is needed across a wide range
How does a serial dilution work?
Produces samples that differ from the next by order of magnitude
Does a muck up in serial dilution concentration impact them all?
Yes, as they move onto later dilutions
What can a colorimeter show?
Concentration of a pigmented solution, turbidity of a liquid, density of cells in a culture
What does a colorimeter do?
Measures the light a sample transmits or absorbs
How does a colorimeter work?
Passes a light beam through the sample and measures the intensity of the light that reaches the detector
How do you use a colorimeter?
Select appropriate wavelength
Set to zero with blank solution (contains no solute)
Replace test cuvette with the sample solution
Record absorb an e
