Topic 4 - Immunocytochemistry

Question 1

antigen

Answer 1

foreign protein

Question 2

antibody

Answer 2

higly specific molecules against antigens

Question 3

Principles of immunity

Answer 3

• Durigng developement the immune system recognices foreign proteins as antigens
• This evokes an immune process
• immune response; production of antibodies
• antibodies binds with high affinity to antigens
• Immunocytochemistry utilizes these antibodies for the localization of tissue components

Question 4

Production of antibodies

Answer 4

1. A tissue constituent is extracted from the body of X animal, and pureified.
2. Injected into bloodstream of another animal. Where it works as an antigen and evokes an immune process, production og antibodies.
3. The antibodies can be extracted from the blood, purified and characterized.

Question 5

Preperation of tissue for immunohistochemistry

Answer 5

1.Collection of sample
Fixation:
Immersion
Perfusion through heart.

Question 6

Perfusion

Answer 6

• Deep anaesthesia
• Cannule introduced through the left ventricle or into the aeorta
• wash out the blood with saline
• fix with paraformaldehyde og glutealdehyde
• remove wanted tissue or organ
• immersion fixed for some hours
• sectioning
• incubation

Question 7

Immunofluoroscence

Answer 7

Antigen-antibody binding in the tissue

Antigenantibody binding between primary and secondary antibody labelled with fluroescent dye