Topic 2 - Special microscopes Flashcards
Definition;
LM developed for special diagnoctics, or reaserch purposes
Types of special microscopes;
- Stereo M
- Dark field M
- Phase contrast M
- Polarization M
- Fluroscence M
- Confocal scanning M
- Colposcope
Stereo M
Used to increase resolution of specimens visible with naked eye.
Surgical M = Stereo M with moving stage
Dark filed M
W&W + Usage
Reade, 1840
Demonstration of non-stainable bacteria
Phase contrast M
W&W + Usage
Zernika, 1936
Study of non-stained, even living preparates
(ex:cell cultures)
Polarization M
W&W + Usage
Talbot, 1829
For membranes, cross-striated muscles and diagnosing illnesses with crystal formation
Fluroscense M
W&W + Usage
Reichert, 1911.
Studying autofluroescence or fluroescence immunocytochemistry
Confocal scanning
Laser light source.
Scans surface of specimen
Emitted light is detected and used to form computerized image. Allows optical section og whicker samples and 3D reconstruction of tissue components.
Use: Study thicker sections and 3D reconstruction
Colposcope
W&W + Usage
Hinselman, 1926
Used for examination of vagina and cervix
