Topic 1 - The light microscope Flashcards
1
Q
Who created the first light microscope and when?
A
Janssen and Janssen, 1590
2
Q
Description of the first LM
A
2m long copper tube with a couple of convexs lenses magnification x60
3
Q
Who was Marcello Malphighi?
A
The first scientist to use the LM for scientific reaserch
4
Q
What is the resolution(Ernst Abbe´s formula)?
A
D=0,6λ/Nsinα D= resolution Lambda = Wavelength N= refraction index α= Apperture angle of lens
Smaller D=More details
Sin α = Always smaller than 1
5
Q
What are the Mechanical parts?
A
Base, Stage, Specimen Stage, Movable holder, tube, Macrometer (course focus) and Micrometer (fine focus)
6
Q
What is the base of stability?
A
Illumination system (mirror,lamp+condenser) Stage holds everything else
7
Q
What does the reflecting prism do?
A
Puts light beam through the specimen
8
Q
What does the Ocular lens do?
A
It is movable to fit eye line of the user
9
Q
What does the Ocular and objective lens do?
A
Produces an enlarged reverse image
