The Prokaryotes (Eubacteria and Archeaea Flashcards

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1
Q

What are prokaryotes? (What kingdoms do they encompass?)

A

Single cell oragnisms.

Lack a nucleus, because they do not have membrane bounded organelles.

Typically the smallest organismsm on earth.

Kingdoms:
Eubacteria and Archea

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2
Q

What are the importance of prokaryotes? (There harmful effects on organisms)

A

-> Can cause many diseases in humans and in other organisms (From the perspective of the infected, it is harmful, from the perspective of the bacteria/prokaryote it is benefitual)
–> How is it benefitual for the prokaryote (Benefit environment, more food, etc,)
–> For certain prey (animals) it can be benefitual, because if the predators were attacked or infected with disease, they pray would live another day.

——-> Infection bacterias are considered pathogens (disease-causing agents, usuaing a virus or micro-organismsm [in this case a micro-organissm])
—> Affects more than humans, such as crops and live stocks.
—> By affecting one part of the food chain/kingdom, causes a domino affect.
–> Threatens our food supply.

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3
Q

How are prokaryotes benefitual towards the environment?

A

-> play a key role in recycling nutrients, as many prokaryotes act as decomposers or producers (Breaking down things to rebuild and reuse)
–> Help out with vital biogeochemical cycles such as the nitrogen cycle (Keeping things in balence)

-Photosynthetic bacteria (which belong in eubacteria and archea) are majour producers of atmospheric oxygen in marine environments.

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4
Q

How are prokaryotes benefitual towards humans?

A

-> Within the intestine of animals and humans (ie: ecoli, produces vitamin K and B12)
-Which is an example of mutalism and symbiosis

-Essenital to food production (yogurt, butter, cheese)
-Produces antibiotics (Can kill/weakan micro-organismsm)
-Usses and produces medically valuable componds (ie: insulin and human growth)
-Seweage treatment
-Odour control
-Digests organic matter and waste
—–> Within the human digestive system.

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5
Q

Whats the difference between antibiotics and vaccines?

A

Antibiotics fight bacterial infections, while vaccines fight viral infections.

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6
Q

What is eubacteria?

A

A kingdom, known as bacteria.
There are 6 different types of eubacteria with different features. However there are 8 main componets of the bacteria.

1) Prokaryotic
(Unicellular)
(No membrane bounded organnelles)

2) DNA structure = single loop, found in a region called a nucleoid.
–> The single loop is different from a humans DNA strand (not a double helix shape)
–> Not encomposed in a membrane or shell, freely flowing but is within an area (usually clumped up)

3) Has plasmids (one or many in its a cytoplasm)
–> Small loops of DNA, that contain a small # of genes, and is used to move around (used for reproduction)
–> These genes are not essential for cell function, however gives an advantage for cells.

4) Has ribosomes that are scattered throughout the cytoplasm (crutial for protein synthesis)

5) Has “pilli”
–> Similiar to villi and cillia
–> Help the cell attach to different surfaces and cells
–> Made up of stiff proteins

6) Has 1 or more flagellas
Tail-like structure fore movement.

7) Has a cell wall, made out of peptidoglycan
–> A large molecule that forms long chains, these chains are crossed linked, making the cell wall strong and rigid.

8) Has a capsule.
–> An outerlayer that provides protection for cells.
–> Why? The cells are very tiny, therefore needs as much protection as possible.
- SImilar to a membrane, helps reduce water loss and exposure to high temps.

9) All can reproduce asexually by binary finnision, some can reproduce sexually by conjunction

10) Mainly autotropic, some are heterotrophic
11) DOES not contain chloroplasts.

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7
Q

The structure of a typical bacteria cell?

A

Cell wall encompasses the layer of peptidoglycan and the cell wall, the outer wall has pilli, and plasmid is apart from the nucleoid.

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8
Q

What are the 3 types of bacterial shapes?

A

Cocci (singular - coccus) -Round
Bacilli (Singular - bacillus) -Rod shape (cylindar)
Spirill (Singular - Spirillum) -Spiral shape

All of these different shapes serve different functions.

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9
Q

Whats the function of the cocci/coccus shape?

A

-Resists drying

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10
Q

Whats the function of the bacilli/bacillus shape?

A

Allows the bacteria to absorb more nutriends due to greater surface area from the shape.

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11
Q

Whats the function of the Spirilli (Spirillum?)

A

Moves through fluids with the least resistance, “snake like”

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12
Q

When identifying bacteria, what are the prefixes used to group them/name them?

A

Prefix diplo-
Arranged in pairs

Prefix staphylo-
Arranged in clusters (like grapes)

Prefix strepto-
Arranged in chains/rod shaped
streptococcus pyogenes - strep throat

If the bacteria is on its own, use its singular name, NOT its plural name.

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13
Q

What are the two ways to identify bacteria?

A

Classifying bacteria VIA there cell wall
Classifying bacteria VIA respiration.

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14
Q

What is the Gram Stain?

A

Hans Gram discovered a method to classify bacteria, highlights the basic differences in the molecular arrangement in bacteriaal cell walls VIA dying the bacteria.

Differenciates between two majour cell wall types (Gram Negative) and (Gram Positive)
–> Method only works for eubacteria.

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15
Q

What does Gram positive bacteria mean?

A

Gram positive = contains more layers of peptidoglycan in the cell wall, staining purple.

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16
Q

What does Gram negative bacteria mean?

A

Gram negative, there is less layers of peptidogylcan in the cell walls.
Stains Pink or red.

–> Bacteria is still eubacteria, it just has less peptidoglycan.

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17
Q

Main differences between Gram + and Gram - ?

A

Gram +: Thick peptidogylcan layer, with no outer lipid membrane.

Gram -: Thin peptidoglycan layer, and a outer lipid membrane.

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18
Q

Classifying bacteria VIA respiration, what are the two main categories?

A

1) Aerobic bacteria
- Bacteria that can survive and grow in oxygenated environments (“Air a Robic”)
-
2) Anaerobic bacteria
-Bacteria that does not need oxygen to survive and grow
(ANA)

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19
Q

What is a obligate aerobe?

A

An organism that cannot survive without oxygen (An extreme)
-> Always needs it, ie: plants and animals.

20
Q

What is an obligate anaerobe?

A

An organism that cannot survive IN the present of oxygen, to them oxygen is “Toxic”

21
Q

What is a faculutative anaerobe?

A

An organism that can live in the presence of oxygen, but does not require it, it doesnt need oxygen, however it does not bother the organism.

Note!! Some anerobic bacterias can switch from to using oxygen when it becomes available. (An extreneply positive adaptation)

22
Q

What are the types of aerobic and anaerobic bacterias?

A

Obligate aerobe, Obligate anaerobe, Faculative anaerobe.

23
Q

Metabolism and bacteria, what can eubacteria be? (In terms of receiving their food?)

A

-Autotrophs (Make their own food)
-Chemotrophs (A subcategroy from autotrophs, but they make their own nutrients/energy from chemicals)

-Heterotrophs (Obtain energy from eating other organismsm [ie; plants and animals])

24
Q

Autrophic organismsm (in comparasion to inorganic and organic chemicals)

A

Make complex carbon molecules from inorganic chemicals (Non living things) (CO2, H20, Mineeralls)

25
Q

Heterotrophic organismsm (compared to organic and inorganic materials)

A

Aquire food from carbon containing organic matierals/chemicals
–> Any materials that contain carbon is produced by living things / any remains of living things.

26
Q

What are cyanobacteria?

A

bacteria that is capable of photosynthesis
–> Considered as microorganismsm
THESE ARE NOOT PLANTS!
-Prokaryotic and unicellular

27
Q

What is chemotrophic bacteria in depth?

A

-> Make their own energy from chemicals, such has hydrogen or sulfer,

–> Create this energy by oxidizing (Putting materials with oxygen) inorganic chemicals (Not from living things)

28
Q

What are the two types of reproduction bacteria can do?

A

Asexual reproduction and sexual reproduction

Asexual
-Genetically indifferent
- No fusion of gamates
- Same number of chromosomes

Sexual
-Genetically differnet
-Fusion of gamates

29
Q

What is binary fission?

A

Aexual reproduction that all eubacteria cells can do
–> Produces 2 daughter cells that are gentically the same.
- Same number of chromosomes
- Same number of plasmids
- A Single strand of DNA replicates (Therefore creating 2 sets of genetes that are each transfered to a new cell) (Then to create the cell, a cell wall froms between the two cells

–> Reproduces extremely fast

30
Q

What are the steps of binary fission?

A
  1. Replication of DNA:

The single, circular chromosome of the bacterium replicates itself.
The two copies of the chromosome move to opposite ends of the cell.
2. Cell Growth:

The cell elongates and increases in size.
New cell membrane and cell wall material are synthesized.
3. Cytokinesis:

A septum (partition) forms in the middle of the cell, dividing the cytoplasm.
The cell wall grows inward, completing the separation of the two daughter cells.

  1. Formation of Two Daughter Cells: (Seperation)

The cell splits into two identical daughter cells, each with a complete copy of the original DNA.

31
Q

By producing really fast, are there any ecological effects or consquences?

A

–> Can be “invasive”
-> Ie: Algal blooms within large bodies of water, which reduces oxygen within the water.

32
Q

What are the two sexual methods of bacteria reproducing?

A

Conjugation and Transformation: (This increases genetic diversity)

33
Q

Other than sexual reproduction how else can genetic diversity be increases in bacteria?

A
  • Mutations (Introduces new genetic info)
  • Viruses infecting bacteria (and injecting viral DNA into them )
  • Transdunction, a process where viruses accidentally pic up DNA from their bacteria/host, and when that virus spreads, the new host hets the DNA from the hold host (in reference to a specific virus that affecs bacteria called: Bacteriophages)
34
Q

What is conjugation?

A

-Two cells joining together to exchange genetic info! method of horizontal gene transfer

How?
1. Donor cell + recipipant cell attach to each other with pillius (Hair like structure for attachement, similar to villi and cillia)
–> Donor cell has a plasmid (Moveable, circular piece of DNA, a part from the nucleoid)
2. Cells make contacts with each other
3. DNA (plasmids) is cut at a specific point and is transfered to the recpiant cell through the pilius
—> Both the donor and recipant has the strand of DNA, as they both replicate.
4. Then the cells seperate, eaching carrying the plasmid DNA

35
Q

How does transformation work?

A

Bacterial transformations, where bacteria takes up foreign DNA from its surroundings.

Dna is released into the environment when bacteria dies and breaks open. some bacteria has specialized proteins on their surface to bind to free bacteria and transport into cells.

When the bacteria takes this new DNA, it recombines with its own DNA and the donors DNA, which create genetic diversity.

However if DNA from a different speicies is floating around, that is called horizontal gene transfer (1DNa from One speces into another species)

36
Q

What are endospores?

A

Structures that surround the bacteria cell, and help the bacteria survive.

They are highly resistant structures, that withstand harsh condiditions. Forms when the cell is under high amount of stress, and makes the cells dormant (all metabolic process and growth shuts down, until conditions improve)
–> Open up and allows the cell to reactive
–> Bacteria is able to live for thousands of years.

37
Q

What are bacterial diseases ?

A

Diseases that range in severity (Ear infections —> Bubonic plagues)

-Can be causes diseases by releasing and producing toxins
or if the bacteria releases toxins upon cell death.

38
Q

Expand/what is an example of a bacteria that cause diseases by releasing or producing toxins?

A

-> Clostridium Botinunum, a bacteria that feowins in non-persevered foods, which causes botulin, a disease that causes muscles paralysis.

39
Q

Expand/examples of bacterias thase cause diseases by releasing toxins upon cell death.

A

—> STrains of ecoli, ie: when ecoli is killed by the immune system/response (gastric juices) it releases toxins which cause severe food posoning

40
Q

Antibiotics and antiresistant bacteria?

A

When antibiotics are used too often or incorrectly, they kill the bacteria that is susceotable to the drug, but the bacterial with natural resistance either due to a mutaton or inherited genes, suvle and multiple, and overtime they become non resistant.

41
Q

Archaea? What is it and how is it different Eubacteria?

A

–> Live in extreme conditions (Extremely hot, extremely cold, and acidic)

-> There cell wall is more resistant to physical and chemical distruptions because it is not made out of peptiodoglycan, but rather glycoproteins. There are 5 types of archaea bacterias.

-Thermophilies
-Methanogens
-Halophiles
-Acidophiles
-Pyschrophiles

42
Q

Thermophiles archeae bacteria.

A

Habitat: extremely hot, ie volcanoes
Source of energy: Sulfur
Oxygen requirement: Anaerobic or faculative Anaerobic
CHaracrerstistcs: Tolerate extreme heat or acidity

43
Q

Methanogens?

A

Habitat: oxygen free encironments or low oxygen (ie swamps, etc)
Source of energy: ]generates energy by converting chemical compounds into methan gas
Oxygen requirement: Obligate Anaerobic
CHaracrerstistcs: perdouces methane gas as a byproduct of metabolism

44
Q

Halophiles?

A

Environment: Salty (ie dead sea)
Energy source: organic food molescules and light
Oxygen requirement. mostly aerobic

45
Q

Acidophiles/Alkliphiles

A

Habitat: Acidic or basic environments
Energy: Dependent on specific speicies
Oxygen requirement: Varies

46
Q

Psychrophiles

A

-Cold environments (Antartica, etc)
Energy: Varies
Oxygen requirement: Varies.

47
Q

what are endospores?

A

A dormant structure that forms inside certain bacteria in response to stress;
Protects the cell’s chromosome from damage

Forms around chromosome when cell is under stress.
environment is not good for growth and survival
Highly resistant structure - Withstand extreme conditions
Remain dormant until conditions improve (often for years)
Protect the cell until environment improves
Open up and allow the cell to reactivate