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Pharmacy (year one) > Sterilisation > Flashcards

Sterilisation Flashcards

1
Q

What is sterilisation?

A

The killing or removal of all viable organisms within a growth medium

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2
Q

What is inhibition?

A

Effectively limiting microbial growth

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3
Q

What is decontamination?

A

The treatment of an object to make it safe to handle

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4
Q

What is disinfection?

A

Directly targets the removal of all pathogens, not necessarily all microorganisms

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5
Q

What is meant by the decimal reduction time?

A

The amount of time required to reduce viability tenfold

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6
Q

Endospores can be killed with heat. True or false?

A

False - can resist heat

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7
Q

What are the methods of heat sterilisation?

A

Red heat
Dry heat
Autoclaving

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8
Q

What is autoclaving?

A

Steam under pressure

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9
Q

Autoclaving doesn’t allow temp of water to exceed 100 degrees. True or false?

A

False

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10
Q

What is pasteurisation?

A

Process of using precisely controlled heat to reduce the microbial load in heat-sensitive liquids

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11
Q

In autoclaving - it is the pressure that kills the microorganisms. True or false?

A

False - it is the high temp

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12
Q

Pasteurisation kills all organisms. True or false?

A

False - that’s why it is different to sterilisation because it doesn’t kill all

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13
Q

UV has sufficient energy to cause modifications and breaks in DNA. True or false?

A

True

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14
Q

UV is useful for decontamination of surfaces. True or false?

A

True

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15
Q

What is ionising radiation?

A

Electromagnetic radiation that produce ions and other reactive molecules

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16
Q

Some microbes are resistant to radiation. True or false?

A

True

17
Q

Radiation is used for the sterilisation of food both in the UK and US. True or false?

A

False - only in US

18
Q

Filtration avoids the use of heat on sensitive liquids and gases. True or false?

A

True

19
Q

What is the pore size in filtration?

A

0.22um

20
Q

Filtration requires either push or pull. What can be used for push and what can be used for pull?

A

Push - pressure

Pull - vacuum

21
Q

What is cold sterilisation used for?

A

Decontaminate heat sensitive materials e.g. thermometers

22
Q

Cold sterilisation uses volatile, toxic, gaseous chemicals such as….

A

Ethylene oxidde
Formaldehyde
Peroxyacetic acid
Hydrogen peroxide

Pharmacy (year one) (60 decks)

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