Special stains and cultures

Question 1

Silver stain

Answer 1

H. pylori, Pneumocystis, Legionella

Question 2

Tellurite agar

Answer 2

C. diphtheria

Question 2

M. TB

Answer 2

Lowenstein-Jensen agar

Question 2

Green metallic sheen on MacConkey agar

Answer 2

E. coli

Question 4

Answer 4

Cryptococcus india ink prep

Question 5

Fungi

Answer 5

Sabouraud agar

Question 6

Answer 6

Partially acid fast oocysts of cryptosporidium

(diarrhea in AIDS)

Question 6

Thayer-Martin media

Answer 6

Neisseria spp

Question 7

Bordetella pertussis

Answer 7

Bordet-Gengou (potato) agar

Question 8

What does Thayer-Martin media contain and why is it used?

Answer 8

Vancomycin (kills G+), polymyxin (kills G- except Neisseria), nystatin (kills fungi)

Isolate N. gonorrhae or meningitidis

Question 9

Neisseria spp

Answer 9

Thayer-Martin media

Question 10

MacConkey agar

Answer 10

Lactose-fermenting enterics turn pink

(E. coli, Klebsiella)

Question 11

Answer 11

Legionella silver stain (Gram - intracellular coccobacillus)

Question 11

Needs NAD+ and hematin

Answer 11

H. flu

(Factors V and X, respectively)

Question 11

Lactose-fermenting on MacConkey agar

Answer 11

E. coli, Klebsiella

Question 12

Sabouraud agar

Answer 12

Fungi

Question 13

C. diphtheria

Answer 13

Tellurite agar, Loeffler medium

Question 13

Lowenstein-Jensen agar

Answer 13

M. TB

Question 14

Loeffer medium

Answer 14

C. diphtheria

Question 16

Answer 16

TB acid fast

(nubs = TB; long = Nocardia)

Question 17

Answer 17

P. falciparum Giemsa stain

Question 17

Requires cysteine and iron

Answer 17

Legionella

(provided on charcoal yeast extract)

Question 19

Answer 19

Borrelia Giemsa

(spirochete! Lyme disease)

Question 21

What does PAS stain?

Answer 21

Glycogen and mucopolysaccharides

Question 22

Cryptococcus

Answer 22

India ink prep

Question 23

H. pylori, Pneumocystis, Legionella

Answer 23

Silver stain

Question 24

Answer 24

Chlamydia Giemsa stain

(Intranuclear inclusions)

Question 24

H. flu

Answer 24

Chocolate agar, contains factor V (NAD+) and X (hematin)

Question 26

Bordet-Gengou (potato) agar

Answer 26

Bordetella pertussis

Question 28

What is the one important use of PAS?

Answer 28

Whipple’s disease

(diarrhea, abdominal pain, joint pain)

Question 29

Eaton agar

Answer 29

Mycoplasma pneumoniae

(Contains cholesterol)

Question 31

Answer 31

Nocardia acid fast

(long = Nocardia; nubs = mycobacterium)

Question 33

Answer 33

Trypansomiasis Giemsa

Question 34

Answer 34

Pneumocystis silver stain

(Flattened footballs)

Question 35

Needs cholesterol

Answer 35

Mycoplasma pneumoniae

Question 36

Ziehl-Neelsen

Answer 36

Acid fast stain

Mycobacterium, Nocardia, oocysts of Cryptosporidium

Question 37

Answer 37

T. whippeli PAS

(Large reddish blobs in GI mucosa)

Question 38

Chocolate agar

Answer 38

H. flu

Question 38

Mycoplasma pneumoniae

Answer 38

Eaton agar (contains cholesterol)

Question 39

Non-lactose fermenting on MacConkey agar

Answer 39

Pseudomonas, Shigella, Proteus

Question 40

Answer 40

H. pylori silver stain

(notice GI mucosa)

Question 42

Charcoal yeast extract agar

Answer 42

Legionella

(Buffered with cysteine and iron)

Question 43

Legionella

Answer 43

Charcoal yeast extract

(Contains cystiene and iron)

Question 44

Gram - enterics

Answer 44

MacConkey agar to know if they’re lactose-fermenting (E. coli, Klebsiella) or non-lactose-fermenting (Proteus, Pseudomonas, Shigella)

Question 45

Giemsa stain

Answer 45

Chlamydia, Rickettsia, Borrelia, Plasmodium, Trypanosomes

Question 46

India ink

Answer 46

Cryptococcus

Question 47

Answer 47

Rickettsia Giemsa stain

(Morulae of Erlichiosis)