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Step One R&M > Special stains and cultures > Flashcards

Special stains and cultures Flashcards

1
Q

Silver stain

A

H. pylori, Pneumocystis, Legionella

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2
Q

Tellurite agar

A

C. diphtheria

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2
Q

M. TB

A

Lowenstein-Jensen agar

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2
Q

Green metallic sheen on MacConkey agar

A

E. coli

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4
Q
A

Cryptococcus india ink prep

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5
Q

Fungi

A

Sabouraud agar

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6
Q
A

Partially acid fast oocysts of cryptosporidium

(diarrhea in AIDS)

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6
Q

Thayer-Martin media

A

Neisseria spp

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7
Q

Bordetella pertussis

A

Bordet-Gengou (potato) agar

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8
Q

What does Thayer-Martin media contain and why is it used?

A

Vancomycin (kills G+), polymyxin (kills G- except Neisseria), nystatin (kills fungi)

Isolate N. gonorrhae or meningitidis

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9
Q

Neisseria spp

A

Thayer-Martin media

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10
Q

MacConkey agar

A

Lactose-fermenting enterics turn pink

(E. coli, Klebsiella)

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11
Q
A

Legionella silver stain (Gram - intracellular coccobacillus)

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11
Q

Needs NAD+ and hematin

A

H. flu

(Factors V and X, respectively)

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11
Q

Lactose-fermenting on MacConkey agar

A

E. coli, Klebsiella

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12
Q

Sabouraud agar

A

Fungi

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13
Q

C. diphtheria

A

Tellurite agar, Loeffler medium

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13
Q

Lowenstein-Jensen agar

A

M. TB

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14
Q

Loeffer medium

A

C. diphtheria

16
Q
A

TB acid fast

(nubs = TB; long = Nocardia)

17
Q
A

P. falciparum Giemsa stain

17
Q

Requires cysteine and iron

A

Legionella

(provided on charcoal yeast extract)

19
Q
A

Borrelia Giemsa

(spirochete! Lyme disease)

21
Q

What does PAS stain?

A

Glycogen and mucopolysaccharides

22
Q

Cryptococcus

A

India ink prep

23
Q

H. pylori, Pneumocystis, Legionella

A

Silver stain

24
Q
A

Chlamydia Giemsa stain

(Intranuclear inclusions)

24
Q

H. flu

A

Chocolate agar, contains factor V (NAD+) and X (hematin)

26
Q

Bordet-Gengou (potato) agar

A

Bordetella pertussis

28
Q

What is the one important use of PAS?

A

Whipple’s disease

(diarrhea, abdominal pain, joint pain)

29
Q

Eaton agar

A

Mycoplasma pneumoniae

(Contains cholesterol)

31
Q
A

Nocardia acid fast

(long = Nocardia; nubs = mycobacterium)

33
Q
A

Trypansomiasis Giemsa

34
Q
A

Pneumocystis silver stain

(Flattened footballs)

35
Q

Needs cholesterol

A

Mycoplasma pneumoniae

36
Q

Ziehl-Neelsen

A

Acid fast stain

Mycobacterium, Nocardia, oocysts of Cryptosporidium

37
Q
A

T. whippeli PAS

(Large reddish blobs in GI mucosa)

38
Q

Chocolate agar

A

H. flu

38
Q

Mycoplasma pneumoniae

A

Eaton agar (contains cholesterol)

39
Q

Non-lactose fermenting on MacConkey agar

A

Pseudomonas, Shigella, Proteus

40
Q
A

H. pylori silver stain

(notice GI mucosa)

42
Q

Charcoal yeast extract agar

A

Legionella

(Buffered with cysteine and iron)

43
Q

Legionella

A

Charcoal yeast extract

(Contains cystiene and iron)

44
Q

Gram - enterics

A

MacConkey agar to know if they’re lactose-fermenting (E. coli, Klebsiella) or non-lactose-fermenting (Proteus, Pseudomonas, Shigella)

45
Q

Giemsa stain

A

Chlamydia, Rickettsia, Borrelia, Plasmodium, Trypanosomes

46
Q

India ink

A

Cryptococcus

47
Q
A

Rickettsia Giemsa stain

(Morulae of Erlichiosis)

Step One R&M (38 decks)

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