Sorting Motifs/prokaryotic v. eukaryotic protein sorting

Question 1

What are the four requirements of sorting into cellular compartments?

Answer 1

1) Sorting Motif Recognition 2) transport with the aid of chaperones 3) movement into or across a membrane 4)association of preproteins with chaperones

Question 2

Inner membrane sorting motif in bacteria

Answer 2

one of the only sorting motifs in bacteria; location of the signature is at the amino terminus; +/hydrophobic/polar

Question 3

ER sorting motif

Answer 3

LOS: amino terminus; TOS: +/hydrophobic/polar

Question 4

Nuclear import sorting motif

Answer 4

LOS: amino terminus; TOS: +/PKKKRKV

Question 5

Nuclear export sorting motif

Answer 5

LOS: amino terminus; TOS: leucine rich (LQLPPLERLTL)

Question 6

Mitochondrial sorting motif

Answer 6

LOS: amino terminus; +/amphiphillic a-helices

Question 7

Peroxisomes sorting motif

Answer 7

LOS:carboxy-terminus; TOS: SKL

Question 8

ER retention sorting motif

Answer 8

LOS: carboxy terminus TOS:4

Question 9

Lysosomes and endosomes sorting motif

Answer 9

LOS: carboxy-terminus TOS: KDEL

Question 10

N-terminus sorting sequences

Answer 10

are often cleaved after their job is done