Basic Overview of generation of siRNAs and miRNAs and their mode of action

Question 1

1

Answer 1

(siRNA) derived from dsRNA comes from virus or lab ; (miRNA) derived from nuclear pre-miRNA

Question 2

2

Answer 2

Dicer complex - cleaves dsRNA into smaller version

Question 3

3

Answer 3

Dicer transfers RNAs to risc

Question 4

4

Answer 4

Risc- separates small dsRNA into two strands. 1 strand is degraded 1 is left over

Question 5

5

Answer 5

Risc guides small RNAs to mRNAs in the cell that are homologous (high sequence identity)

Question 6

Argonaute proteins

Answer 6

use small RNAs as guides to find complementary mRNA

Question 7

6 Function of miRNA

Answer 7

Not exact complementary (translationally inhibits mRNA)

Question 8

6 Function of siRNA

Answer 8

has good complementary; binds mRNAl perfect pairing (Uses Risc’s nuclease activity cutting mRNA)

Question 9

Function of Dicer

Answer 9

Endoribonnuclease; forms miRNA and siRNA; facilitates the formation of RISC

Question 10

Risc

Answer 10

RNA-induced silencing complex ; bind ss siRNA and miRNA