Glycosylation in the ER

Question 1

Be sure to….

Answer 1

go to pages 17 and 18

Question 2

Why is glycosylation important?

Answer 2

1) increases solubility of proteins 2) prevention of inappropriate protein aggregation 3) small monosaccharides are involved in cell signaling 4) protect secreted proteins from degradation 5) cell recognition

Question 3

Process of glycosylation

Answer 3

1) N-acetylglucosamine (GlcNAC) and mannose residues added together and translocated in the ER 2) Soluble glucose added in the ER to GlcNAc and mannose 3) enzyme transports glycosyl group to asparagine group onto protein 4) modifications to glycoslyations

Question 4

Purpose of removing glucose 1 by 1

Answer 4

causes chaperones to recognize glycosylations, bind to it, and halt folding until entire molecule is produced.

Question 5

N-linked glycosylations

Answer 5

carbohydrates added to amino end of asparagine

Question 6

O-linked glycosylations

Answer 6

addition of carbohydrates on OH end of serine