RNA transcription Flashcards
Leader and tailing sequence
Immediately following the inverted methylated base 5’ cap.
Followed by start codon
Non translated
Immediately after the the stop codon
Followed by Poly A tail
Non translated
Hairpins
.
Start and Stop Codons
AUG - Start
UAG - Stop
UAA - Stop
UGA - Stop
RNA Polymerase
.
Holoenzyme
prokaryotic RNA poly enzyme consists of various subunits that comprise the holoenzyme
Core Enzyme
RNA poly subunits without Sigma subunit
Sigma Subunit
Recognizes the promoter regions
Amanitin (Alpha-Amantin)
mushroom poison
used to distinguish eukaryotic RNA polymerases
Rifampicin
antibiotic
used to distinguish eukaryotic RNA polymerases
Primary Transcript
pre-rRNA
Mature Transcript
pre-rRNA after modifications
- removal of both external and internal nucleotides by ribonucleases
- base modification
- addition of nucleotides.
Snurps
.
Ribonucleases
.