Rhabdoviruses

Question 1

Virus in the family of Rhabdovirus

Answer 1

Rabies

Question 2

Genus of Rabies

Answer 2

Lyssavirus

Question 3

Physical features of rabies

Answer 3

• capsid helical
• enveloped
• bullet shaped

Question 4

Nucleic acid of rabies

Answer 4

negative ; single stranded ; RNA

Question 5

Location of replication of rabies

Answer 5

cytoplasm

Question 6

Replication cycle of rabies

Answer 6

1. bind to cell surface via Proteins
2. virus penetrates host cell, enters by pinocytosis
3. virus enters endosome
4. transcription; viral polymerase transcribe 5 mRNA and + RNA templates
   5,6. Translation; synthesis of N,P,M,G,L proteins
5. Replication; viral polymerase makes copies of +RNA which act as templates for -RNA.
6. Assembly; NPL complex+ (-)RNA + M proteins
7. Bud

Question 7

Rabies is a disease of the

Answer 7

CNS

Question 8

Incubation period

Answer 8

20-90 days; asymptomatic

Question 9

Prodromal phase of rabies

Answer 9

• similar to flu

- discomfort at bite

Question 10

Neurological phase

Answer 10

• 2 to 7 days
• cerebral dysfunction, anxiety, confusion, agitation
• acute confusion, abnormal behavior, coma, cardiac arthymia

Question 11

Rabies mechanism of transport in body

Answer 11

Travel via retrograde axonal transport in CNS; then transport to eye, skin, salivary glands via nerves

Question 12

Rabies virus is concentrated in;

Answer 12

Salivary glands

Question 13

Rabies and its association with hydrophobia

Answer 13

Invades and damages the muscles involved in drinking and swallowing; suffer excruciating pain on swallowing liquids.

Question 14

Transmission and common reservoir of rabies

Answer 14

• bites and scratch

- bats,foxes,raccoons, skunk

Question 15

Diagnosis of rabies

Answer 15

-histopathology, electron microscopy, direct florescent antibody test, immunohistochemistry

Question 16

Histopathologic evidence of rabies encephalomyelitis in brain tissue and meninges

Answer 16

1. mononuclear infiltration
2. perivascular cuffing of lymphocytes
3. lymphocytic foci
4. babes nodules of glial cells
5. negri bodies

Question 17

Control of rabies

Answer 17

• administering of vaccine following bite; IgG antibodies

- successive administering of IgG antibodies