Papillomaviridae

Question 1

Genome of Human papilloma virus

Answer 1

• double stranded
• DNA
• circular

Question 2

Capsid structure

Answer 2

• icosahedral
• L1 and L2
• L1: major capsid protein
• L2: minor capsid protein

Question 3

HPV replications and virion assembly occurs in

Answer 3

host cell nucleus

Question 4

Locations in body where replications occurs

Answer 4

• differentiated skin

- mucosa epithelium

Question 5

Papilllomaviruses infect which species

Answer 5

Infect only humans

Question 6

Categories of papillomaviruses

Answer 6

Cutaneous or mucosal

Question 7

Cutaneous HPV presentation on skin

Answer 7

• HPV1-4
• wart
• benign, self limited proliferation of skin
• regress without therapy
• recurrence is common

Question 8

Incubation period of HPV

Answer 8

3 to 4 months

Question 9

Mucosal HPV presentation in body

Answer 9

1. benign head and neck tumors
   - Single oral papillomas
   - epithelial tumors
   - removed by surgery, rare recurrence

• Laryngeal papillomas
• HPV6-11
• removed by surgery, possible recurrence

Question 10

Genital warts location

Answer 10

Condylomata acuminata, HPV6-11

• external genitalia
• perianal areas

Question 11

Transmission of genital warts

Answer 11

sexual contact

Question 12

Cervical dysplasia and Neoplasia cause by HPV types

Answer 12

mainly HPV types 16, 18, 31, 45

Question 13

Characteristic of cervical carcinoma

Answer 13

contain integrated HPV DNA

Question 14

Normal Pathogenesis of cervical carcinoma and HPV

Answer 14

E2 binds to early promotor and decreases expression of E6/E7

Question 15

E6 and E7 proteins are

Answer 15

oncogenic

Question 16

Process of E6 and E7

Answer 16

Bind to and inactivate p53 and RB genes which are cellular growth suppressor genes

Question 17

Outcome of infection by HPV

Answer 17

• productive (lytic) cycle

- latent infection

Question 18

Cells of what layer invaded by HPV

Answer 18

Cells of the basal layer of the epidermis

Question 19

Progression of HPV in the cells

Answer 19

1. latent viral phase no signs or symptoms, mnths-yrs
2. production of viral DNA and proteins
3. Late gene expression, structural proteins, vegetative DNA in terminally differentiated cells of epidermis
4. host cells infected; atypical koilocytosis

Question 20

Review slides of HPV replication

Answer 20

Slides go into more detail

Question 21

Epidemiology of HPV

Answer 21

• transmission via surfaces
• direct contact
• sexual intercourse
• birth

Question 22

Diagnosis of HPV

Answer 22

Koilocytotic cells: cytology
Viral nucleic acid: DNA probe, PCR, southern blot
Viral structural antigens: immunofluorescent/immunoperoxidase staining
Virus: electron microscopy

Question 23

Control of HPV

Answer 23

• cryosurgery, podophyllin resin, podofilox lotion
• aldara; immune system stimulator
• vaccination

Question 24

Vaccination of HPV components

Answer 24

• Use of capsid protein L1, which self assembles into VLPs
• VLPs of 6, 11, 16, 18
• trigger an antibody response