Adenovirus

Question 1

Structure composition of core protein hemagglutinin

Answer 1

Penton base + fiber + knob domain

Question 2

Genome of adenovirus

Answer 2

• linear
• double stranded
• DNA

Question 3

Capsid of adenovirus

Answer 3

Icosahedral

Question 4

Is it enveloped?

Answer 4

No

Question 5

Replication process of adenovirus

Answer 5

1. Binds to CAR receptor
2. Alphav integrins binds to penton base on adenovirus
3. internalization of adenovirus via endocytosis

Question 6

Transcription, replication, and assembly takes place

Answer 6

Nucleus of infected cell

Question 7

Two phases of transcription, and divided by?

Answer 7

Early and late transcription, divided by DNA replication

Question 8

Polymerase used for mRNA synthesis?

Answer 8

host cell RNA polymerase

Question 9

Early mRNAs transcribed from which regions

Answer 9

E1, E2, E3, E4

Question 10

Intermediate early genes?

Answer 10

E1A and E1B

Question 11

Genes transcribed following E1A and E1B

Answer 11

E2, E3, E4

Question 12

Early genes are involved in

Answer 12

1. DNA synthesis
2. Alter expression of host cell genes
3. Transcription of late genes(L1-L5)

Question 13

Functions of early mRNAs

Answer 13

1. Block CTL response
2. modulate cell cycle
3. block apoptosis
4. shut down mRNA host transport
5. DNA replication of progeny DNA

Question 14

Functions of late mRNAs

Answer 14

1. Virion assembly

2. Cell lysis

Question 15

Clinical presentation of adenovirus

Answer 15

1. respiratory tract disease
2. ocular disease
3. acute hemorrhagic cystitis
4. gastroenteritis
5. infections in immunosuppressed

Question 16

Symptoms of respiratory tract disease

Answer 16

• fever, rhinorrhea, cough, sore throat
• prolonged fevers, leukocytosis, bacterial infections
• lower respiratory tract infections, conjunctivitis, bronchitis

Question 17

Symptoms of ocular diseases

Answer 17

1. pharyngoconjuctival fever

2. Epidemic keratoconjuctivitis

Question 18

Pathogenesis of adenovirus

Answer 18

1. transmitted by droplet inhalation - resp. infection

2. transmitted by fecal oral route - GI infection

Question 19

Viral processes that adenovirus can undertake

Answer 19

1. Lytic infection
2. Latent infection - lymphoid tissues
3. Oncogenic transformation studies in rats

Question 20

Epidemiology of Adenovirus

Answer 20

• common in infants and children

- rare in immunocompetent individuals

Question 21

Control

Answer 21

• no treatment

- prevention with live attenuated vaccine; discontinued

Question 22

Patients susceptible to adenoviral infections

Answer 22

• allogenic stem cell transplantation
• t cell depletion
• lymphopenia
• neutropenia

Question 23

Location that is damaged by adenovirus during lytic infection

Answer 23

• mucoepithelial cells in
• RT
• GI tract
• conjunctiva or cornea

Question 24

Adenovirus can resist what

Answer 24

-drying
-detergents
-GI tract
-chlorine
Possible since a naked virus