Perfecting Microscope Skills

Question 1

also called a stereoscopic microscope

Answer 1

dissecting microscope

Question 2

includes both scanning and transmission

Answer 2

electron microscope

Question 3

light microscope

Answer 3

compound microscope

Question 4

Name all the microscopes used to examine specimens and objects difficult or impossible to see with the naked eye

Answer 4

dissecting, electron, and compound microscope

Question 5

used to view the surface of relatively larger specimens at low power typically no more than 10x, functions like elaborate magnifying glasses

Answer 5

dissecting microscope

Question 6

one would use this type of microscope to observe gross anatomy of living, preserved or dichotomize a specimen at greater detail.

Answer 6

dissecting microscope

Question 7

scientific instruments that use a beam of highly energetic electrons to examine objects on a very fine scale (up to several 100,000)

Answer 7

electron microscopes

Question 8

a microscope that is limited by the physics of light 500x or 1000x magnification

Answer 8

compound microscope

Question 9

consist of two lens and the associated hardware to make viewing of specimens easier

Answer 9

compound microscope

Question 10

The upper most lens of the compound microscope is called the and is the part through which a person looks

Answer 10

ocular lens

Question 11

The lower lens on the compound microscope is called

Answer 11

the objective lens

Question 12

usually several are mounted on a turret, allow rapid change

Answer 12

objective lens

Question 13

lens on the compound scope used in lab are

Answer 13

4x scanning power, 10x low power, and 40x high power

Question 14

holds the ocular and objective lenses in place

Answer 14

body tube

Question 15

Most microbiological specimens are mounted on ______ _____ with _____ _____ on top and placed on the ______.

Answer 15

glass slides, cover slips, stage

Question 16

holds the slide firmly on the stage

Answer 16

clips or clamps

Question 17

A ______thus the name light microscope and a ______ _____ are located beneath the stage

Answer 17

light source, condenser lens

Question 18

focuses the light through a hole in the stage and usually has an iris

Answer 18

condenser

Question 19

varies the amount of light passing through the specimen

Answer 19

iris

Question 20

After passing through the specimen, the light goes through the

Answer 20

objective and ocular lenses and then into the eye of the observer

Question 21

the image is calculated by multiplying the magnification of the ocular by the magnification of the objective

Answer 21

total magnification

Question 22

Why is a compound microscope called a compound

Answer 22

because it has two lenses

Question 23

As light passes through any object it bends. This bending is called

Answer 23

refraction

Question 24

the measurement of the extent that the substance bends light is called

Answer 24

the refraction index of a substance

Question 25

causes distortion of the image

Answer 25

excessive refraction

Question 26

at magnifications of less than 500x

Answer 26

the distortion is minimal

Question 27

helps remedy the problem with distortions

Answer 27

oil immersion

Question 28

topmost series of lenses through which an object is viewed

Answer 28

ocular lens or eyepiece

Question 29

holds nosepiece at one end and eyepiece at the other end; conducts light rays

Answer 29

body tube

Question 30

supports upper parts and provides carrying handle

Answer 30

arm

Question 31

Objective lenses scanning power objective

Answer 31

holds 4x lens used to view the whole slide

Question 32

Objective lenses low-power objective

Answer 32

holds 10x lens used to view the object in greater detail

Question 33

objective lenses high-power objective

Answer 33

holds 40x lens used to view the object in even greater detail

Question 34

holds 100x lens and is used in conjunction with immersion oil to view objects

Answer 34

oil immersion

Question 35

knob used to being object into approximate focus; used only with low-power objective

Answer 35

coarse-adjustment knob

Question 36

knob used to being object into final focus

Answer 36

fine-adjustments knob

Question 37

controls amount of illumination used to view the object

Answer 37

diaphragm or diaphragm control lever

Question 38

an attached lamp that directs a beam of light up through the object

Answer 38

light source

Question 39

the flat surface of the microscope that rests on the table

Answer 39

base

Question 40

hold and supports microscope slides

Answer 40

stage

Question 41

a moveable stage that aids in the accurate positioning of the slide

Answer 41

mechanical stage

Question 42

two knobs that are usually located below the stage. One knob controls forward/reverse movement, and the other controls right/left movement

Answer 42

mechanical stage control knobs

Question 43

State the rules for Microscope Use

Answer 43

1). The lowest power objective should be in position both at the beginning and end of microscope use (scanning objective) 2). Use only lens paper for cleaning lenses. 3) Do not tilt the microscope when viewing a wet mount 4) Keep the stage clean and dry to prevent rust and corrosion 50 Do not remove parts of the microscope 6) Keep the microscope dust-free by covering it after it after use. 7) Report any malfunctioning when you notice it. This will benefit you and those who use the microscopes after you

Question 44

How do you focus on the Microscope

Answer 44

1) always start at the lowest power objective lens 2) turn the nose piece so that lowest power lens is in straight alignment over the stage 3) alway begin focusing with lowest power objective lens 4) with the coarse-adjustment know, lower the stage until it stops

Question 45

once the object is in focus with lowest power (scanning power) it should be nearly in focus with higher power

Answer 45

parfocal