NPNs: Uric Acid

Question 1

Major Product of Purine Catabolism

Answer 1

Uric acid

Question 2

Purine is made from

Answer 2

Guanine + Adenine (PURGA)

Question 3

Pyrimidine

Answer 3

pCUT

Pyrimidine = Cytosine, Uridyl, Thymine

Question 4

Formed from xanthine oxidase from liver and intestine

Answer 4

Uric acid

Question 5

Uric acid exist as _________ urates in plasma

Answer 5

Monosodium urates

Question 6

> 6.8 g of urate crystals may form

Answer 6

Precipitate in tissues (gout arthritis)

Question 7

Organic Meat
Legumes
Plant

Answer 7

High Purine Food

Question 8

Confirms and Monitor Gout

Answer 8

Uric acid

Question 9

diagnosis of Renal Calculi using Uric Acid uses what specimen

Answer 9

Urine

Question 10

Uric acid can be used to monitor Uric Acid Nephropathy in cancer patient that undergoes chemotherapy

Answer 10

Yes

Question 11

Uric Acid Crystals:

Reference Value
Male
Female

Answer 11

Male 3.5-7.2 mg/dL
Female 2.6-6.0 mg/dL
Urine 250-750 mg/day

Question 12

Gout
Increased Nuclear Metabolism
Chronic Renal Disease
Lesch Nyhan Syndrome

Answer 12

Hyperuricemia

Question 13

• Occurs in male between 3rd and 5th decade of life
• Negative birefringence crystals in synovial fluid
• acute inflammatory arthritis
• can lead to nephrothiliasis

Answer 13

Gout

Question 14

Seen in leukemia, Lymphoma, MM and PV, Hemolytic and Megaloblastic Anemia

Treatment: Allupurinol

Answer 14

Increased Nuclear Metabolism

Question 15

Decreased GFR and tubular secretion
BUA >10 mg/dL

Answer 15

Chronic Renal Disease

Question 16

Inborn Error of Purine Metabolism

Answer 16

Lesh-Nyhan Syndrome

Question 17

Orange sand diaper syndrome ; deficiency of HGPT (Hypoxanthine Guanin Phosphoribosyl Transferase

Answer 17

Lesh-Nyhan Syndrome

Question 18

Seen in patient with Preeclampsia

Answer 18

Inc. BP
Inc. Protenuria
Inc. Uric Acid

Question 19

Treatment for Hyperuricemia:

Inhibits xanthine oxidase activity ; lowering UA in serum without affecting excretion in kidney

Answer 19

Allopurinol

Question 20

Treatment for Hyperuricemia:

Drugs that lower the serum UA but increase excretion of UA in urine = Prone to uric acid stones if not maintained at Alk pH.

Answer 20

Uricosuric drugs (Probenecid and Sulfinpyrazone)

Question 21

Treatment for Hyperuricemia:

1. Neither excretion nor production is affected
2. Alters phagocytic effect of leukocytes in uric acid crystals in tissues
3. used in symptomatic patients

Answer 21

Colchicine

Question 22

1. Fanconi Syndrome
2. Wilson’s disease
3. Hodgkin’s disease
4. Overtreatment of Allopurinol
5. Chemotherapy with azathioprine / 6-mercaptopurine
6. Liver Disease

Answer 22

Hypouricemia

Question 23

Laboratory Methods for Uric Acid:

Chemical Method that uses
Principle: Redox Reaction

Answer 23

Caraway Method / Phosphotungstic Acid Method

Question 24

Laboratory Methods for Uric Acid:

Product produced in Caraway Method / Phosphotungstic Acid Method

Answer 24

Tungsten BLUE + ALLANTOIN + CO2

Question 25

Laboratory Methods for Uric Acid:

Enzymatic Method for Uric Acid (Blauch and Koch) uses the principle of

Answer 25

UV Spectrophotometry

Question 26

Laboratory Methods for Uric Acid:

Enzymatic method used in Uric Acid

Answer 26

Blauch and Koch

Question 27

Laboratory Methods for Uric Acid:

In Enzymatic Method for Uric Acid (Blauch and Koch) measures the ; product (?)

Answer 27

Decreased in absorbance at 293 nm

Question 28

Laboratory Methods for Uric Acid:

In Enzymatic Method for Uric Acid (Blauch and Koch) uses enzyme

Answer 28

Uricase

Question 29

Specimen used for Uric Acid Tests:

Answer 29

Heparinized Plasma
Serum or Urine

Question 30

Specimen used in Uric Acid Tests are stable for at

Answer 30

3 days ; Room Temperature

Question 31

Interference in Uric Acid Tests:

LBH
Lipemia, Bilirubin, Hemolysis

Answer 31

False Decrease

Question 32

Interference in Uric Acid Tests:

Salicylates and thiazides

Answer 32

False Increase