Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

CLINICAL CHEMISTRY > LIPIDS: Lipoproteins II > Flashcards

LIPIDS: Lipoproteins II Flashcards

You may prefer our related Brainscape-certified flashcards:
Periodic Table flashcards Chemistry 101 flashcards AP® Chemistry flashcards Organic Chemistry 101 flashcards
1
Q

Major protein found in HDL ; activates LCAT; ANTIATHEROGENIC

A

Apo A1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Major Protein found in LDL ; Risk of CHD

A

Apo B100

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Independent risk factor ; Impaired Plasminogen Activation

A

Lipoprotein a

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

LABORATORY METHODS FOR LIPOPOROTEINS

A

Ultracentrifugation
Electrophoresis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Laboratory Method for Lipoproteins:

Based on Density and Shape
Sample is adjusted with Sodium Bromide and is centrifuged at high speed for 24 HOURS

A

Ultracentrifugation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Laboratory Method for Lipoproteins:

Reference method for Lipoproteins

A

Ultracentrifugation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Laboratory Method for Lipoproteins:

Order from MOST TO LEAST DENSE (ULTRACENTRIFUGATION)

A
  1. HDL (bottom)
  2. LDL
  3. VLDL
  4. Chylomicrons (Top;creamy layer)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Laboratory Method for Lipoproteins:

Separation based on size and charge

A

Electrophoresis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Laboratory Method for Lipoproteins:

Stains for Electrophoresis for Lipoprotein

A

Oil Red O
Sudan Black B
Sudan III
Sudan IV
Fat Red B

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Laboratory Method for Lipoproteins:

Arrangement from fastest/most anodic (Electrophoresis)

A

HDL > LDL > VLDL > CM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Laboratory Method for Lipoproteins:

Test primarily used for Chylomicrons; Involves standing aliquot of plasma in Refrigerator for 4 deg celsius overnight

A

Standing Plasma Test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Laboratory Method for Lipoproteins:

Standing Plasma Test: The aliquot remains turbid even after standing overnight

A

Excess BVLDL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Laboratory Method for Lipoproteins:

Standing Plasma Test:

Presence of Chylomicrons at a Fasting Plasma

A

Abnormal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Laboratory Method for Lipoproteins:

Polyanion Precipitation (3-step Precipitation Method)

Homogenous Assay

These test are used for the measurement of ?

A

HDL Measurement

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Laboratory Method for Lipoproteins:

In Polyanion Precipitation (3-step Precipitation Method):

Agents used to precipitate Lipoprotein with Apo B

A

Dextran Sulfate
Heparin Sulfate
Phosphotungstate

Divalent cation:
Magnesium
Calcium
Manganese

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Laboratory Method for Lipoproteins:

In Polyanion Precipitation (3-step Precipitation Method):

Reagents used

A

Heparin sulfate - Manganese Chloride
Dextran Sulfate - Magnesium Chloride

16
Q

Laboratory Method for Lipoproteins:

In Polyanion Precipitation (3-step Precipitation Method):

HDL supernatant is measured using (2)

A

Cholesterol Oxidase
Cholesterol esterase

17
Q

Laboratory Method for Lipoproteins:

In Polyanion Precipitation (3-step Precipitation Method):

Can be automated T/F

A

FALSE

POLYANION CANNOT BE AUTOMATED

18
Q

Laboratory Method for Lipoproteins:

Used for HDL measurement; uses antibody to bind with Apo B100 (VLDL, LDL)

HDL then is measured ENZYMATICALLY

Principle is used in automated measurement

A

Homogenous Assay

19
Q

Laboratory Method for Lipoproteins:

  1. Friedawald Equation
  2. Delong Equation
  3. Beta Quantification
  4. Homogenous Direct LDL Method
  5. Gel Chromatography / Affinity Chromatography
  6. Immunochemical
  7. Immunoassay or immunonephelometry
A

LDL Measurement

20
Q

Laboratory Method for Lipoproteins:

LDL Measurement suitable if TRG is <400

A

Friedwald Equation

21
Q

Laboratory Method for Lipoproteins:

LDL = TC - (HDL + VLDL)
VLDL = TRG / 5 (g/dL)
VLDL = TRG / 2.175 (mmol/L)

A

Friedwald Equation

22
Q

Laboratory Method for Lipoproteins:

LDL measurement used when TRG is >400 g/dl

A

Delong Equation

23
Q

Laboratory Method for Lipoproteins:

LDL = TC - (HDL + VLDL)
VLDL = TRG / 6.5 (g/dL)
VLDL = TRG / 2.825 (mmol/L)

A

Delong Equation

24
Q

Laboratory Method for Lipoproteins:

LDL measurement used in Research ; requires the used of Ultracentrifugation

A

Beta Quantification

25
Q

Laboratory Method for Lipoproteins:

  1. Ultracentrifugation ( 18 hrs at 105 k x g) separate VLDL and CM
  2. Remaining solution –> Measure Chole
  3. Precipitate LDL —> Measure Chole in Sln
  4. Difference of the 2 measurement is the concentration of LDL
A

Beta Quantification

26
Q

Laboratory Method for Lipoproteins:

Useful when TG is elevated (>600 md/dL)

A

Homogenous Direct LDL Method

27
Q

Laboratory Method for Lipoproteins:

Uses 2 reagent
First Reagent: remove non-LDL
Second Reagent: releases cholesterol from LDL to be measured enzymatically

A

Homogenous Direct LDL Method

28
Q

Laboratory Method for Lipoproteins:

Immunoassay / Immunonepholometry

Acceptable CV:

TG

A

5 %

29
Q

Laboratory Method for Lipoproteins:

Immunoassay / Immunonepholometry

Acceptable CV:

TC

A

3 %

30
Q

Laboratory Method for Lipoproteins:

Immunoassay / Immunonepholometry

Acceptable CV:

HDL

A

4%

31
Q

Laboratory Method for Lipoproteins:

Immunoassay / Immunonepholometry

Acceptable CV:

LDL

A

4%

32
Q

Provides evidence-based guidelines for cholesterol testing and management, CHD risk asssessment

A

NCEP
National Cholesterol Education Program

33
Q

recognize additional positive risk for CHD, Lp(a), hs-CRP, preexisting subclinical atherosclerosis

A

ATP III
Adult Treatment Panel III

34
Q

Can be subdivided into 2:

  1. Hyperlipoprotenimia
  2. Hypolipoprotenimia
A

Dyslipidemia

35
Q

Classified by Frederickson-Levy Classification System

A

Hyperlipoprotenimia

36
Q

Hyperlipoprotenimia can be subdivided into (3)

A
  1. Hypercholesterolemia
  2. Hypertriglyceridemia
  3. combined hyperlipidemia

CLINICAL CHEMISTRY (29 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions