Automation Flashcards
It is a technique used to measure current or voltage produced by the activity of an ion
Electrochemistry Technique
What are the 5 techniques under Electrochemistry Technique
- Potentiometry
- Amperometry
- Coulometry
- Polarography
- Voltammetry
It measures the VOLTAGE potential at a constant current
Potentiometry
Potentiometry uses (2)
pH
pCO2
Potentiometry Reference Electrode (2)
- Calomel
- Silver-silver Chloride
Two types of Potentiometry (2)
- Direct Ion Selective Electrode
- Indirect Ion Selective Electrode
It measures the DISSOLVED ELECTROLYTES at fluid phase
Ion Selective Electrode
Ion Selective Electrodes depends on the (?)
Barrier/Membrane
What are Barrier/Membrane used by ISE (6)
Vice Ganda GONE
1. Vancomycin Gel (Potassium)
2. Glass Aluminum Silicate (Sodium)
3. Gas
4. Organic Ion Liquid exchanger (Calcium and Lithium)
5. Nonactin and Monactin
6. Enzymes
Interferences condition happens in ISE
Protein-coating ISE > Build-up counter-voltage > defective ISE
Differences of Direct and Indirect ISE
DIRECT ISE
- w/o sample dilution
- not prone to PSEUDOHYPONATREMIA encountered in hyperlipidemic and hypoproteinemia sample
INDIRECT ISE
- w sample dilution
It is a electrochemical titration that uses amperometry as a detector
COULOMETRY
Coulometry measure amount of ____ at fixed potential
electricity
Coulometry uses (1)
Chloride Test
Chloride Test sample spx (3)
CSF
Serum
Sweat