Musculoskeletal histology Flashcards

1
Q

what are the differences between cells, tissue and organs

A

cell: single, basic unit of life
Tissue: group of cells working together
Organ: group of tissues working together to provide a bodily function

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2
Q

what colours do different structures appear when stained with romanowsky stain

A
  • nucleus/nucleoproteins = blue/purple
  • cytoplasmic granules = blue or orange
  • cytoplasm = red/orange
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3
Q

what colours do different cellular structures appear when stained with haematoxylin and Eosin

A
  • nucleus/nucleoproteins = blue/purple
  • cytoplasmic granules = blue or orange
  • cytoplasm/collagen/protein = red/orange/pink
  • cartilage = pale blue
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4
Q

what is methylene blue stain used for

A
  • injected into tissue planes to delineate neoplasms

neoplasm: abnormal growth of cells

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4
Q

what is methylene blue stain used for

A
  • injected into tissue planes to delineate neoplasms

neoplasm: abnormal growth of cells

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5
Q

what is fluorescein stain used for

A
  • IV to identify devitalised tissue (colic surgeries)
  • Eye ulcers
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6
Q

what is immunohistochemistry

A

the process of detecting antigens in a tissue by using antibodies to understand the distribution and localization of different antigens

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