mouse immunodeficiency models

Question 1

what mutation is present in nude mice?

Answer 1

Foxn1 nu

Question 2

what mutation is present in SCID mice?

Answer 2

Prkdc scid

Question 3

deficiencies seen in nude mice

Answer 3

decreased T cells, but leaky

Question 4

deficiencies seen in SCID mice?

Answer 4

decreased T and B cells, but leaky

Question 5

deficiencies seen in SCID beige mice

Answer 5

decreased T, B and NK cell activity

Question 6

deficiencies seen in NOD-SCID mice

Answer 6

decreased T, B, and NK activity, minimal leaky

Question 7

deficiencies seen in NSG, or NOG mice

Answer 7

decreased T, B and NK activity, not leaky

Question 8

what are the common medical complications seen in nude mice?

Answer 8

females fail to lactate
susceptible to Corynebacterium bovis
susceptible to Staph xylosus
ocular issues
lack of whiskers

Question 9

what chromosome is the SCID mutation on?

Answer 9

Chr 16

Question 10

what immunodeficiency is seen in the SCID mouse?

Answer 10

defective DNA dependent kinase coding for TCR and B cell receptors

Question 11

what chromosome is the Rag mutation on?

Answer 11

Chr 2

Question 12

what chromosome is the Beige mouse mutation on?

Answer 12

Ch 13

Question 13

what is the cause of the immunodeficiency seen in the Beige mouse?

Answer 13

lysosomal storage disease

Question 14

what is the cause of the immunodeficiency seen in the XID mouse?

Answer 14

defective Bruton’s tyrosine kinase (signal transduction in B cells)

Question 15

what immunodeficiency seen in the moth eaten mouse?

Answer 15

lacks cytotoxic T and NK cells

Question 16

what is the cause of the immunodeficiency seen in the moth eaten mouse?

Answer 16

defective phosphatase, Chr 6

Question 17

list two mouse models for systemic lupus erythematosus

Answer 17

NZB x NZW F1 hybrid

FAS lpr mouse

Question 18

what chromosme is the Foxn1 (nude mouse) mutation on?

Answer 18

Chr 11

Question 19

pathophysiology of immunodeficiency in nude mide

Answer 19

dysgenesis of thymic epithelium, lack measurable T cell activity, increased NK cell activity

Question 20

major uses of nude mice

Answer 20

oncology, xenografts

Question 21

What immunodeficiency is seen in the Rag1 or 2 knockouts?

Answer 21

Antibody and T cell deficient, not leaky

Question 22

What chromosome is the Rag 1 and 2 found on?

Answer 22

Chr 2

Question 23

breeding of Foxn1nu animals

Answer 23

breed nude males with heterozygous females

Question 24

aside from the NZB/NZW F1, what is another model for SLE

Answer 24

Fas(lpr) on chromosome 19, leads to failure of lymphocytes to undergo apoptosis