misc cards Flashcards
list some rat eplipsy models
1) GAERS rat: genetic absence eplipesy rat
2) GEPR 3 and 9 rats, genetically epilepsy prone rat 9 more severe than 3
3) WAG/Rij
immunodeficiency viruses are in what family?
lentiviruses
what are anabolic steroids classified as by the DEA?
class III
who must qualify researchers and approve protocols using Schedule 1 substances?
the FDA
how long should records be retained for a GLP study that was submitted to the FDA
2 years following approval or 5 years following submisstion
how long should records be retained for a GLP study that was not submitted to the FDA?
2 years from the date the study was completed, discontinued or terminated
what does CITES stand for
convention on international trade in endangered species of wild fauna and flora
CITES appendix I classification description
species threatened with extinction
CITES appendix II classification description
species not necessarily threatened with extinction, but in which trade must be controlled in order to avoid utilization incompatible with their survival
CITES appendix III classification description
species that are protected in at least one country
what is required to acquire a CITES appendix 1 animal
import and export permits
what is required to acquire a CITES appendix 2 animal?
export permit
what is required to acquire a CITES appendix 3 animal from a protected country?
export permit
what is required to acquire a CITES appendix 3 animal from a non-protected country?
certificate of origin
what form is required to register with the DEA
Form 224
what form is required to order schedule 1-2 controlled substances?
Form 222
how long must physical records pertaining to controlled substances be retained?
2 years
how often must inventories of controlled substances be performed?
every 2 years
what must a controlled substance inventory contain
date on which inventory was conducted, names of the controlled substances, dosage units and types number of dosage units number of commercial containers record of disposition
define schedule 1 controlled substances
have a high potential for abuse, have no currently accepted medical use, lack of accepted safety for use of the drug or other substance under medical supervision
define schedule 2 controlled substances
high potential for abuse which may lead to severe psychological or physical dependence
define schedule 3 controlled substances
potential for abuse less than substances in schedules 1 and II and abuse may lead to moderate or low physical dependence or high psychological dependence
define schedule 4 controlled substances
low potential for abuse and may lead to limited physical dependence or psychological dependence
define schedule 5 controlled substances
low potential for abuse and consist primarialy of preparations containing limited quantities of certain narcotics
pentabarbital is what schedule
II, (III if has phenytoin)
morphine is what schedule
II
medatomidine is what schedule
not controlled
midazolam is what schedule
IV
thiopental is what schedule?
III
oxymorphone is what schedule?
II
diazepam is what schedule?
IV
Telazol is what schedule?
III
Fentanyl is what schedule?
II
Butorphanol is what schedule?
IV
ketamine is what schedule?
III
phenobarbital is what schedule?
IV
buprenorphine is what schedule?
III
xylaxine is what schedule?
not controlled
anabolic steroids are what schedule?
III
chloral hydrate is what schedule?
IV
list some nonhuman primates that are CITES appedix 1
all great and lesser apes, Macaca silensus, Callithrix flaviceps, Saguinus bicolor, S. leacopus, S. oedipus. Saimiri oerstedti
how are narcotic substances regulated
by CFR title 21 part 1300 controlled substance act, administered by the secretary of health and human services adn enforced by the DEA
what is the agency responsible for importation of all animals/animal products that include animals or products that could pose a disease threat to US livestock, infectious organisms and vectors, cell cultures and recombinant products?
USDA, APHIS
what is the document for transportation regulations?
CFR49 title 9, chapter 1
what animals require USDA permits for import
swine, ruminants, other hoof stock, poultry/birds
which small lab animals require USDA permits?
animals that have been inoculated/exposed to infectious agents. trangenic animals that are specifically made to allow for productive infection with human pathogens.
which agency controls import of non human primates?
us public health service through the CDC, office of health and safety
how often must importers register with the CDC?
every 2 years
who is responsible for regulations pertaining to importing animals that may be infectious to humans?
PHS, US dept of health and human services
who is responsible for enforcement of regulations regarding transport of endangered species
US fish and wildlife service, department of the interior
what does the Lacey Act cover?
transportation, importation, exportation, sale or purchase of any fish, wildlife or plants that may harm humans or US horticulture, forestry or wildlife resources
what is the governing ordnance for GLP work?
CFR title 21 part 58
irradiation dose for standard rodent feed
25 kGy
irradiation dose for gnotobiotic rodent feed
50 kGy
effect of UV-A black light
320-400nm. pigmentation of skin
effect of UV-B light
280-320nm, photokeratitis, cataracts
effect of UV-C light
100-280nm, germicidal effects
define class I laser
no hazardous radiation
define Class II laser
not enough power to injure someone accidentally, can cause injury if viewed for extended period of time
define Class IIIa laser
can cause injury if viewed with binoculars
define Class IIIb laser
can cause injury if viewed directly
define Clads IV laser
can start fires
major rat allergen
Rat n1, urinary
major mouse allergen
Mouse ml, urinary males secrete more than females
major cat allergen
Fel d1, saliva, skin, hair
major dog allergen
Can f1. saliva, hair skin
major guinea pig allergen
urine is major allergen, also dander and saliva
major rabbit allergen
fur is major allergen, saliva and urine are minor
major bird allergen
IgG mediated hypersensivity pneumonitis
describe Splendoe Hoeppli phenomenon, what type of granules are associated with it?
results from Ag-Ab reaction and concurrent accumulation and incorporation of cellular debris, “Botryoid” granules- sulfur granules that stain thick refractive eosinophilic material arranged in a stellate radiating or asteroid manner
what is the trichrome stain used for?
collagen and muscle fiber
what is the Oil Red O or Sudan Black stain used for?
lipids
what is the congo red stain used for?
amyloid, bi-refringent with polarized light
what is the cresyl violet stain used for?
cell bodies of neurons
what is the luxon fast blue stain used for?
myelin
what is the alizarin red stain used for?
calcium (bone)
what is the prussian blue stain used for?
hemosiderin
what is the Alcian blue stain used for?
mucins (cartilage)
what is the PAS stain used for?
polysaccharides, cell wall
properties of thiopental?
ultra short acting barbiturate
what are the properties of Inactin/Thiobutabarbital?
long acting in rats, good for maintaining renal perfusion
bad in rabbits
barbituate
What are the properties of alpha chloralose
long acting
preserves respiratory and cardiovascular reflexes
poor induction and recovery
what are the properties of Avertin/ tribromoethanol
used in GEM’s
can cause inflammation and peritonitis
unreliable plane of anesthesia
action of benzodiazepines on GABA?
enhance the affinity of the receptors for GABA
action of barbiturates on GABA?
decrease the rate of dissociation of GABA from its receptor
what are the properties of chloral hydrate?
schedule IV controlled substance
sedative and hypnotic agent
causes adynamic ileus
medium duration, light anesthesia with minimal effects on cardiovascular system
what are the properties of urethane?
carcinogenic
long acting
minimal reflex depression
give examples of the denaturants
Quaternary ammonium compounds
phenolics
alcohols
give examples of the reactants
aldehydes
ethylene oxide
give examples of oxidants
halogens (chlorine bleach, chlorine dioxide)
Peroxygens (vaporized hydrogen peroxide, Virkon)
