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Vet Tech Prep > Mosby Microbiology > Flashcards

Mosby Microbiology Flashcards

1
Q

What is the average temperature of an incubator?

A

37 degrees celsius or 98 degrees farenheidt

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2
Q

When are cultures grown?

A

Overnight and held for 48 hours

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3
Q

What is nutritive media?

A

Grows all types of bacteria and some fungi

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4
Q

What is selective media?

A

Grows only certain types of bacteria

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5
Q

What is Differential media?

A

Contains elements that differentiate types of bacteria

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6
Q

How are media plates incubated?

A

Upside down to prevent condensation from dripping onto cultures

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7
Q

What is an agar?

A

Semisolid media

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8
Q

What is a broth?

A

Liquid media

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9
Q

What is a plate?

A

Flat round container of agar

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10
Q

What is a slant

A

Tube of agar that has been allowed to gel at an angle

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11
Q

What is a CNA (Columbia colistin nalidixic acid) agar?

A

Selects for gram positive

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12
Q

What is a MAC (MacConkey II) agar?

A

Selects for gram negative
Differentiates between lactose fermentors (purple) and non-lactose fermenters

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13
Q

What is MH (Mueller-Hintong) agar?

A

Antibiotic sensitivity testing

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14
Q

What is TSA (trypticase soy agar with 5% sheeps blood)?

A

Nutritive medium for cultivation of organisms

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15
Q

What is TSA aka?

A

Blood agar plate

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16
Q

What is DTM (dermatophyte test medium)?

A

Isolates pathogenic fungi

17
Q

What is TSB (Trypticase Soy Broth)?

A

Use in blood cultures

18
Q

What can you detect when gram staining feces?

A
  1. Campylobacter (seagull shapes)
  2. Clostridium (Spore form rods)
  3. Yeast
19
Q

What is an acid fast stain?

A

Similar to gram stain
Mycobacterium

20
Q

What is a catalase test?

A

Staphylococcus is positive
Streptococcus is negative

21
Q

What is a coagulase test?

A

Identifies Staphylococcus

22
Q

What does gram stain do for bacteria?

A

Purple is positive
Pink is negative

23
Q

What is alpha hemolysis?

A

Incomplete hemolysis (agar surrounding colony is green)

24
Q

What is beta hemolysis?

A

Complete hemolysis (agar surrounding colony is clear)

25
Q

What is Gamma hemolysis

A

No hemolysis (agar surrounding colony is unaffected)

26
Q
A

Vet Tech Prep (30 decks)

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