Modified Study EXAM 3 Flashcards
Transcription Factors and Activators
What do they do and why are they needed
Transcription factors are made of proteins that bind to DNA at the promotor to help guide RNA polymerase so that transcription can occur
Activators can increase the speed of transcription
These are needed to speed up transcription. Without them, and with billions of cells this process could take a long time. With these factors it takes about 8 hours
How is mRNA translation regulated
For translation to begin, the ribosome, an RNA-and-protein complex that houses translation must assemble on the mRNA (with the help of “helper” proteins)
Regulation on a protein level
“edits” such as removal of amino acids or chemical modification is needed for the protein to become active (can also change activity and behavior)
How do signals from other cells regulate gene expression
During development, signals determine what a cell becomes. Then they help coordinate growth and daily functions
What is electrophoresis
Electric charge that separates DNA segments by size. Smaller = more movement
Steps of Genetic Engineering
Recombinant DNA contains DNA from 2 or more organisms
A vector is used to carry foreign DNA
Restriction Enzymes cut DNA at specific sites to create sticky ends
DNA ligase is used to join pieces of DNA together
Commonly used Vector?
Bacterial Plasmid
Steps of Polymerase Chain Reaction
Denaturation
Annealing
Extension
What is Denaturation in the Polymerase chain reaction
DNA is heated so it becomes single stranded
What is Annealing in the polymerase chain reaction
Nucleotide primer hybridizes to each of the single DNA strands
What is Extension in the polymerase chain reaction
DNA polymerase adds complimentary bases to each of the single DNA strands
What is the point of the Polymerase Chain Reaction
To create billions of copies of a segment of DNA in a short amount of time
What is a transgenic organism
An organism that has had a gene from another species inserted into its genome
They can produce biotech products such as insulin and vaccinations
Genomics
The study of an entire set of DNA sequences in an organism
Proteonomics
The study of the structure and function of cellular proteins and how they interact to produce traits
Bioinformatics
The use of technology to study the genome and proteome
Who influenced Darwin
Cuvier Malthus Lyell Lamarck Wallace
How did Malthus influence Darwin
He proposed that populations grow faster than the resources they depend on which causes the weakest members to get killed off
How did Lyell influence Darwin
He proposed that geological processes have gradually shaped earths surface, proposing that the earth must be far older than originally thought
How did Lamarck influence Darwin
Proposed species change over time by inheritance of acquired characteristics