Exam 2 Lab 5

Question 1

What is Catalase

Answer 1

An enzyme involved in a degrading reaction

Question 2

What reaction does catalase catalyze

Answer 2

The breakdown of hydrogen peroxide

Question 3

Define Enzyme

Answer 3

Organic catalysts that speed up metabolic reactions

Question 4

Define Substrate

Answer 4

Reactants in an enzymatic chemical reaction

Question 5

Define Product

Answer 5

The end result of a reactant, after a metabolic reaction

Question 6

Define Denature

Answer 6

The loss of shape and function (Usually of a protein)

Question 7

Define Synthesis Reaction

Answer 7

Substrates being joined to form a product

Question 8

Define degradation Reaction

Answer 8

Substrates being broken down into a product

Question 9

Define Active Site

Answer 9

Location where the enzyme and substrate form an enzyme-substrate complex

Question 10

The effect of substrate on enzyme activity

Answer 10

Enzymes only react with certain substrates

EX: catalase and hydrogen peroxide. Without both, a reaction will not happen

Question 11

Effect of Temperature on enzyme activity

Answer 11

Individual enzymes react best within their preferred temperature. If its to hot or cold the enzyme can start breaking down and work work. In our experiment, catalase prefers the cold.

Question 12

Effect of concentration on enzyme activity

Answer 12

The more enzymes, the faster the reaction

Question 13

Effect of pH on enzyme activity

Answer 13

Each enzyme has an optimum pH. Potato catalase’s optimum pH is 7 (Neutral). To high or to low can effect hydrogen bonding and not allow the enzyme to work as it should.