Microscopy Flashcards
What are the parts of a miscroscope?
Detector Objective Specimen Light conditioning system Light source
What are some examples of light conditioning systems?
Kohler illumination
Phase ring
Wollaston prism and polarisers
Filter cubes
What is the sample surrounded by when using a light microscope?
Embedding medium
What do you use for alive light microscope specimens?
Box
What controls the temperature of a light microscope box?
Incubator combined with a precision air heater
Why is temperature so important when using a light microscope?
Changes cause contraction -> change in focus
Why is each box custom designed for the light microscope specimen?
Intricate system of openings and doors for comfortable access to microscope controls and specimen
To increase detail in an object what do you need to do in light microscopy?
Increase contrast
What are light microscopes used for?
Histology
Phase contrast-cell morphology
Timelapse (heart cell differentitation, cell migration)
What are the two ways dye can be attached in fluorescence microscopy?
Antibodies and protein fusion
