M5: Vaginal Secretions (Part 1: Intro-Abnormal Appearance) Flashcards
Familiarize or memorize the reasons why vaginal secretions are tested
- To diagnose PROM (Premature Rupture Of the Membrane)
- Infections (vaginosis, trichomonas)
- Forensic purpose (rape victims)
- Abnormal vaginal discharge or odor
- Pruritus
- Dysuria, and dyspareunia
- secondary to bacterial vaginosis (BV), trichomoniasis, or vulvovaginal candidiasis
Vaginitis
Clinical manifestation in vaginitis which is characterized by vaginal irritation
Pruritis
T or F
Vaginitis is secondary to bacterial vaginosis, trichomoniasis, vulvovaginal cadidiasis
T
What are the (3) non-infectious conditions of vagitinis
- vaginal atrophy
- allergies
- chemical irritation
Identify the clinical significance based on color or symptoms it presents:
white
Normal color
Identify the clinical significance based on color or symptoms it presents:
no odor
Normal
Identify the clinical significance based on color or symptoms it presents:
Fishy odor
Abnormal
Identify the clinical significance based on color or symptoms it presents:
Itchy vagina
Pruritis
Identify the clinical significance based on color or symptoms it presents:
Painful urination
Dysuria
Identify the clinical significance based on color or symptoms it presents:
Pain while having sexual intercourse
Dyspareunia
Identify the clinical significance based on color or symptoms it presents:
Vaginal lining becomes thin
Vaginal atrophy
Identify the clinical significance based on color or symptoms it presents:
- Caused by feminine was
- pH imbalance
Chemical irritation
VAGINAL FLUID TESTING
T or F
In vaginal fluid testing, old specimens are needed
F (fresh specimens are necessary)
VAGINAL FLUID TESTING
What are the (3) microscopic methods in vaginal fluid testing
- Saline wet mount preparation
- Potassium hydroxide (KOH) examination
- Gram stain
VAGINAL FLUID TESTING
Gold standard
a. Saline wet mount preparation
b. KOH preparation
c. Gram stain
d. ALL
e. NOTA
c. Gram stain
VAGINAL FLUID TESTING
Familiarize other methods of testing for vaginal fluid
Litmus pH levels, DNA probe testing, culture, and point-of-care test kit
VAGINAL FLUID TESTING
These (3) parameters are used to diagnose ruptured fetal membranes
- Placental α1-microglobulin(PAMG-1)
- Protein
- Insulin-like growth factor binding protein-1 (IGFBP-1)
VAGINAL FLUID TESTING
This enzyme is used to assess the risk of preterm delivery
Fetal fibronectin enzyme
NORMAL FINDINGS IN VAGINAL SECRETION
Appearance:
Whote, flocculent discharge
NORMAL FINDINGS IN VAGINAL SECRETION
pH
3.8 - 4.2
NORMAL FINDINGS IN VAGINAL SECRETION
Amine (whiff) test
negative
NORMAL FINDINGS IN VAGINAL SECRETION
WBC
rare to 2+
NORMAL FINDINGS IN VAGINAL SECRETION
Lactobacilli
predominant
NORMAL FINDINGS IN VAGINAL SECRETION
Clue cells
absent
NORMAL FINDINGS IN VAGINAL SECRETION
Other cells
absent (except rbc during red ride)
NORMAL FINDINGS IN VAGINAL SECRETION
What other organisms can be present in vaginal secretions?
(2)
other lactobacilli subgroups
occational yeast
VAGINAL FLUID
volume of vaginal fluid collected
0.5 to 1.0 mL of sterile physiological saline
VAGINAL FLUID
Specimen shouild be kept at what temperature
room temperature
reserve the motility of Trichomonas vaginalis and the recovery of N. gonorrhoeae
VAGINAL FLUID
Reasons why vaginal fluid specimen are kept at RT
a. presernve motility of trichomonas vaginalis
b. recovery of neisseria gonnorhea
c. both
d. NOTA
c. both
VAGINAL FLUID
Specimens for T. vaginalis should be examined within?
Within 2 hours of collection
VAGINAL FLUID
C. trachomatis and HSV should be kept at what temperature?
Refrigerated
VAGINAL FLUID
Which is incorrect
C. trachomatis and HSV
a. room temp
b. ref temp to induce overgrowth of normal flora
c. both
d. NOTA
d. NOTA
a. room temp (REF TEMP)
b. ref temp to induce overgrowth of normal flora (PREVENT OVERGROWTH)
VAGINAL FLUID
WHat is added as preservatove for vaginal secretion?
Glycerin
ABNORMAL APPEARANCE
Determine specific infection based on discharge:
Increased Thin, Homogenous White-To-Gray Discharge
BACTERIAL VAGINOSIS (BV)
ABNORMAL APPEARANCE
Determine specific infection based on discharge:
White, “Cottage Cheese-Like” Discharge
CANDIDA INFECTION (CI)
ABNORMAL APPEARANCE
Determine specific infection based on discharge:
Increased Yellow-Green, Frothy, Adherent Discharge
TRICHOMONAS VAGINALIS
ABNORMAL APPEARANCE
Determine specific infection based on discharge:
Yellow, Opaque Cervical Discharge
CHLAMYDIA TRACHOMATIS