F2: CSF (Part 4: Tests - TUBE 2) Flashcards

1
Q

What test/section of laboratory is done in tube 2?

A

Microbiology or Culture and Sensitivity

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2
Q

What are the techniques used in tube 2 (microbiology)

A
  • Stains
  • Culture/PCR
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3
Q

What (4) stains are used

A
  • Direct wet mount
  • Gram staining
  • Acid fast Z-N
  • India ink
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4
Q

(4) stains

  • Detects Cryptococcosis
  • Amebic meningoencephalitis
  • Candida infection
  • Trypanosomiasis
A

Direct wet mount

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5
Q

(4) stains

  • Detects bacteria
  • Fungus
A

Gram

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6
Q

(4) stains

  • Tuberculosis
A

Acid fast Z-N

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7
Q

(4) stains

  • Cryptococcus
A

India ink

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8
Q

(4) stains

T or F

Nowadays, TB is no longer stained and is replaced by using PCR

A

T

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9
Q

Where is Neisseria typically found during an infection?

Intra or Extracellular?

A

Inside host cells (intracellular)

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10
Q

Is Streptococcus pneumoniae found intracellularly during infections?

A

No, it is extracellular

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11
Q

What is the Gram stain result for Neisseria?

A

Gram-negative

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12
Q

What is the Gram stain result for Streptococcus pneumoniae?

A

Gram positive

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13
Q

T or F

Yeast is easy to differentiate from rbc and bubble

A

F

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14
Q

What is a key feature of yeast to differentiate it from rbc and bubbles?

A

Budding

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15
Q

What are the 5 common Agents of Bacterial Meningitis

A
  1. Escherichia coli
  2. Neisseria meningitidis
  3. Strep pneumonia
  4. Haemophilus influenzae
  5. Listeria monocytigenes
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16
Q

5 common Agents of Bacterial Meningitis

  • Most common in the neonate
  • Least common in the elderly
A

Escherichia coli

17
Q

5 common Agents of Bacterial Meningitis

  • Moderately common in both neonates and elderly
A

Strep pneumonia

18
Q

5 common Agents of Bacterial Meningitis

  • Most common in the elderly
  • Least common in neonates
A

Listeria monocytigenes

19
Q

5 common Agents of Bacterial Meningitis

Arrange from most common in neonates to least common in elderly:

Listeria monocytogenes, Haemophilus influenzae, Escherichia coli, Strep pneumonia, Neisseria meningitidis

1 - most common in neonates; 5 is least common in elderly

A

1.Escherichia coli
2.Neisseria meningitidis
3.Strep pneumonia
4.Haemophilus influenzae
5.Listeria monocytigenes

20
Q

5 common Agents of Bacterial Meningitis

Arrange from most common in elderly to least common in neonates:

Listeria monocytogenes, Haemophilus influenzae, Escherichia coli, Strep pneumonia, Neisseria meningitidis

1 - most common in elderly; 5 is least common in neonates

A
  1. Listeria monocytigenes
  2. Haemophilus influenzae
  3. Strep pneumonia
  4. Neisseria meningitidis
  5. Escherichia coli
21
Q

Microbiology

Machine used for tuberculosis and viruses

A

PCR