F2: CSF (Part 4: Tests - TUBE 2) Flashcards
What test/section of laboratory is done in tube 2?
Microbiology or Culture and Sensitivity
What are the techniques used in tube 2 (microbiology)
- Stains
- Culture/PCR
What (4) stains are used
- Direct wet mount
- Gram staining
- Acid fast Z-N
- India ink
(4) stains
- Detects Cryptococcosis
- Amebic meningoencephalitis
- Candida infection
- Trypanosomiasis
Direct wet mount
(4) stains
- Detects bacteria
- Fungus
Gram
(4) stains
- Tuberculosis
Acid fast Z-N
(4) stains
- Cryptococcus
India ink
(4) stains
T or F
Nowadays, TB is no longer stained and is replaced by using PCR
T
Where is Neisseria typically found during an infection?
Intra or Extracellular?
Inside host cells (intracellular)
Is Streptococcus pneumoniae found intracellularly during infections?
No, it is extracellular
What is the Gram stain result for Neisseria?
Gram-negative
What is the Gram stain result for Streptococcus pneumoniae?
Gram positive
T or F
Yeast is easy to differentiate from rbc and bubble
F
What is a key feature of yeast to differentiate it from rbc and bubbles?
Budding
What are the 5 common Agents of Bacterial Meningitis
- Escherichia coli
- Neisseria meningitidis
- Strep pneumonia
- Haemophilus influenzae
- Listeria monocytigenes
5 common Agents of Bacterial Meningitis
- Most common in the neonate
- Least common in the elderly
Escherichia coli
5 common Agents of Bacterial Meningitis
- Moderately common in both neonates and elderly
Strep pneumonia
5 common Agents of Bacterial Meningitis
- Most common in the elderly
- Least common in neonates
Listeria monocytigenes
5 common Agents of Bacterial Meningitis
Arrange from most common in neonates to least common in elderly:
Listeria monocytogenes, Haemophilus influenzae, Escherichia coli, Strep pneumonia, Neisseria meningitidis
1 - most common in neonates; 5 is least common in elderly
1.Escherichia coli
2.Neisseria meningitidis
3.Strep pneumonia
4.Haemophilus influenzae
5.Listeria monocytigenes
5 common Agents of Bacterial Meningitis
Arrange from most common in elderly to least common in neonates:
Listeria monocytogenes, Haemophilus influenzae, Escherichia coli, Strep pneumonia, Neisseria meningitidis
1 - most common in elderly; 5 is least common in neonates
- Listeria monocytigenes
- Haemophilus influenzae
- Strep pneumonia
- Neisseria meningitidis
- Escherichia coli
Microbiology
Machine used for tuberculosis and viruses
PCR
