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AUBF > M3: Analysis Automation (Part 3: Sediment/Cell Analyzers) > Flashcards

M3: Analysis Automation (Part 3: Sediment/Cell Analyzers) Flashcards

1
Q

SEDIMENT/CELL ANALYZERS

  • This type of sediment/cell analyzers provides efficient standardized results
  • markedly improves TAT
A

Automated Microscopy Analyzers

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2
Q

SEDIMENT/CELL ANALYZERS

What are 2 types of technology used in sediment/cell analyzers

A
  • Flow cytometry
  • Digital imaging techniques
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3
Q

What are the automated urine system for sediments/cells?

A
  • Sysmex UF-1000i Fully Automated Urine Particle Analyzer and Sysmex UN-2000 Automated Urinalysis System
  • Beckman Coulterʼs iQ200 Microscopy Analyzer
  • 77 Elektronika UriSed 2 Automated Urine Sediment Analyzer
  • 77 Elektronika UriSed 3Pro Automated Urine Sediment Analyzer
  • FUS-100 and FUS-200 Sediment Analyzers
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4
Q

Automated urine system for sediments/cells

  • Uses fluorescent flow cytometry
  • Measures forward, side, and flourescent signal
  • Has 2 channels
  • uses 1.2 mL of uncentrifuged urine
  • particles are identified by measuring the height and width of the fluorescent and light scatter signals
A

Sysmex UF-1000i Fully Automated Urine Particle Analyzer and Sysmex UN-2000 Automated Urinalysis System

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5
Q

Automated urine system for sediments/cells

In Sysmex UF-1000 and Sysmex UN-2000, it uses flow cytometry to measure what (3) parameters of light

A
  1. Forward light scatter
  2. Side scatter
  3. Fluorescent signal
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6
Q

Automated urine system for sediments/cells

This measures size (length of cells)

Sysmex UF-1000 and Sysmex UN-2000

(3) parameters of light

A

Forward light scatter

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7
Q

Automated urine system for sediments/cells

Measures shape and internal structure/complexity of the element

Sysmex UF-1000 and Sysmex UN-2000

(3) parameters of light

A

Side scatter

Sysmex UR-1000 and Sysmex UN-2000

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8
Q

Automated urine system for sediments/cells

Measures cellular inclusions

Sysmex UF-1000 and Sysmex UN-2000

(3) parameters of light

A

Fluorescent signal

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9
Q

Automated urine system for sediments/cells

What are the requirements in Sysmex UF-1000i and Sysmex UN-2000?

Sysmex UF-1000 and Sysmex UN-2000

A
  • 1.2 mL of uncentrifuged urine
  • 2 channels (sediment and bacterial channel)
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10
Q

Automated urine system for sediments/cells

T or F

1.2 mL of centrifuged urine is used in Sysmex UF-1000i and Sysmex UN-2000

Sysmex UF-1000 and Sysmex UN-2000

A

F (1.2 mL uncentrifuged urine)

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11
Q

Automated urine system for sediments/cells

Channel for urine particle analysis

Sysmex UF-1000 and Sysmex UN-2000

A

Sediment channel

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12
Q

Automated urine system for sediments/cells

Channel for bacteria staining and detection

Sysmex UF-1000 and Sysmex UN-2000

A

bacterial channel

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13
Q

Automated urine system for sediments/cells

This stabilizes the pH and lyses the non-bacterial particles

Sysmex UF-1000 and Sysmex UN-2000

bacteria channel

A

diluent

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14
Q

Automated urine system for sediments/cells

T or F

stain in bacterial channel is specific to the RNA in a bacterial cell

Sysmex UF-1000 and Sysmex UN-2000

A

T

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15
Q

Automated urine system for sediments/cells

Bacterial channel is incubated at what temperature?

Sysmex UF-1000 and Sysmex UN-2000

A

42 c

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16
Q

Automated urine system for sediments/cells

Resulting values of Sysmex UF-1000 and Sysmex UN-2000 are presented in what unit?

Sysmex UF-1000 and Sysmex UN-2000

A

quantitative cells per microliter (μ) and cells per HPF or LPF

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17
Q

Automated urine system for sediments/cells

Main particles detected in Sysmex UF-1000 and Sysmex UN-2000

Sysmex UF-1000 and Sysmex UN-2000

A
  • RBCs
  • WBC
  • SECs
  • Hyaline casts
  • Bactera
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18
Q

Automated urine system for sediments/cells

Flagged particles in Sysmex UF-1000 and Sysmex UN-2000

Sysmex UF-1000 and Sysmex UN-2000

A
  • Pathologic casts
  • Crystals
  • Small round cells (RTE/transitional ECs), sperm, mucus, yeast-like cells
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19
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
Pathologic casts

Sysmex UF-1000 and Sysmex UN-2000

A

flagged particles

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20
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
Crystals

Sysmex UF-1000 and Sysmex UN-2000

A

flagged particles

Sysmex UF-1000 and Sysmex UN-2000

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21
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
RTE cells

Sysmex UF-1000 and Sysmex UN-2000

A

flagged particle

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22
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
Transitional ECs

Sysmex UF-1000 and Sysmex UN-2000

A

flagged particles

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23
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
sperm

Sysmex UF-1000 and Sysmex UN-2000

A

flagged

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24
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
mucus

Sysmex UF-1000 and Sysmex UN-2000

A

flagged

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25
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
yeast-like cells

Sysmex UF-1000 and Sysmex UN-2000

A

flagged

Sysmex UF-1000 and Sysmex UN-2000

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26
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
RBCs

Sysmex UF-1000 and Sysmex UN-2000

A

main particle

27
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
WBCs

Sysmex UF-1000 and Sysmex UN-2000

A

main particle

28
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
SECs

Sysmex UF-1000 and Sysmex UN-2000

A

main particle

29
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
Hyaline casts

Sysmex UF-1000 and Sysmex UN-2000

A

main particle

30
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
Bacteria

Sysmex UF-1000 and Sysmex UN-2000

A

main particle

31
Q

Automated urine system for sediments/cells

Flagged particles indicate the need to perform what (2) procedures?

Sysmex UF-1000 and Sysmex UN-2000

A

Manual microscopic analysis and identification

32
Q

Automated urine system for sediments/cells

T or F

Sysmex UF-1000 and Sysmex UN-2000 can store up to 10,000 results including scattergrams, histograms, and specimen characteristics

Sysmex UF-1000 and Sysmex UN-2000

A

T

33
Q

Automated urine system for sediments/cells

T or F

Sysmex UF-1000 and Sysmex UN-2000 can only be used alone

Sysmex UF-1000 and Sysmex UN-2000

A

F (independently used or integrated with automated urine chemistry strip reader to create a complete urinalysis)

34
Q
  • the principle also used for CBC (specifically, RBC, WBC, and platelet counts)
  • the passage of different urine elements in a single file in front of a laser so they can be detected, counted, and sorted
A

Flow cytometry

35
Q

Components of a flow cytometry

A
  • Laser
  • Fluidic system
  • Optic system
  • Photodetector
  • Computer system
36
Q

Flow cytometry

Process of flow cytometry

A
  1. Some elements are fluorescently-labelled and excited by the laser (emit light at varying wavelengths)
  2. The optic system gathers the light
  3. Detector receives light which converts the received scattered light into a voltage pulse
  4. The computer system converts/transcribes the data into a histogram or scattergram
37
Q

Components of a flow cytometry

Gathers light

A

Optic system

38
Q

Components of a flow cytometry

Converts the received scattered light into a voltage pulse

A

detector

39
Q

Components of a flow cytometry

Converts/transcribes the data into a histogram or scattergram

A

Computer system

40
Q

Components of a flow cytometry

Flow cytometry can transcirbe data into

a. histogram
b. scattergram
c. both
d. NOTA

A

c. both

41
Q

Automated urine system for sediments/cells

  • uses digital flow morphology (imaging) and auto particle recognition (APR) to categorize and count urine particles automatically in uncentrifuged urine based on size, shape, texture, and contrast
  • uses 1.3 mL of urine
  • Used for body fluid cells
  • Digital camera takes 500 images
A

Beckman Coulterʼs iQ200 Microscopy Analyzer

42
Q

Automated urine system for sediments/cells

T or F

Beckman Coulterʼs iQ200 Microscopy Analyzer is not used ofr body fluid cells

A

F (also used)

43
Q

Automated urine system for sediments/cells

T or F

The sample moves in planar flow cell and presented to a digital microscope in a thick moving sheet

Beckman Coulterʼs iQ200 Microscopy Analyzer

A

F (sample moves in planar flow cell and presented to a digital microscope in a thin moving sheet)

44
Q

Automated urine system for sediments/cells

Volume of urine used?

(centrifuged or uncentrifuged?)

Beckman Coulterʼs iQ200 Microscopy Analyzer

A

1.3 mL of uncentrifuged urine

45
Q

Automated urine system for sediments/cells

  • Digital camera takes how many images as sample advances through flow cell

Beckman Coulterʼs iQ200 Microscopy Analyzer

A

500 images

46
Q

Automated urine system for sediments/cells

What sediments does Beckman Coulterʼs iQ200 Microscopy Analyzer classify?

Beckman Coulterʼs iQ200 Microscopy Analyzer

A

RBCs, WBCs, WBC clumps, SECs, non-SECs, unclassified casts, hyaline casts, unclassified crystals, bacteria, yeast, mucus, and sperm

subclassifications also exist in 27 more categories

47
Q

Automated urine system for sediments/cells

  • performs automated microscopy with digital imaging using auto image evaluation module
    (AIEM) software
  • Uses 2mL of centrifuged urine, bright field microscope
  • Analyzed by bright-field microscope
  • a digital camera captures and categorizes 15 particle images based upon size and shape using AIEM software
  • Zoom capability to view images
A

77 Elektronika UriSed 2 Automated Urine Sediment Analyzer

48
Q

Automated urine system for sediments/cells

T or F

77 Elektronika UriSed 2 Automated Urine Sediment Analyzer’s interpretation is like that of automatic microscopic smears

A

F (interpretation is like that of manual microscopic smears)

49
Q

Automated urine system for sediments/cells

  • incorporates phase contrast microscopy in addition to bright-field microscopy to improve differentiation of elements such as hyaline casts, RBC membranes, crystals, and yeasts
A

77 Elektronika UriSed 3Pro Automated Urine Sediment Analyzer

50
Q

Automated urine system for sediments/cells

What can be linked with 77 elektronika urised 2 and 3pro to make complete automated urinalysis laboratory system

A

LabUMat 2

LAB U

51
Q

Automated urine system for sediments/cells

  • flat-flow cell digital imagine technology
  • artificial imaging identification (AII) software classifies and quantifies the cells and formed particles in the uncentrifuged urine
  • a digital camera captures up to 820 photos
A

FUS-100 and FUS-200 Sediment Analyzers

52
Q

Automated urine system for sediments/cells

classifies and quantifies the cells and formed particles in the uncentrifuged urine

FUS-100 and FUS-200

A

artificial imaging identification (AII) softwarre

53
Q

Automated urine system for sediments/cells

Centrifuged or uncentrifuged urine?

FUS-100 and FUS-200 Sediment Analyzers

A

uncentrifuged urine

54
Q

Automated urine system for sediments/cells

digital camera captures to how many photos?

FUS-100 and FUS-200 Sediment Analyzers

A

820 photos

it identifies and classifies 12 visible components in urine

55
Q

Automated urine system for sediments/cells

How many visible components in urine are identified and classified?

FUS-100 and FUS-200 Sediment Analyzers

A

12

56
Q

Automated urine system for sediments/cells

What can be integrated with FUS-100 and FUS-200 Sediment Analyzers for a fully automated urinalysis system

FUS-100 and FUS-200 Sediment Analyzers

A

H-800 chemistry analyzer

57
Q

Zoom capability

a. Beckman’s Coluter iQ200 microscopy analyzer
b. Sysmex UF-1000 and Sysmex UN-2000 automated urinalysis system
c. both
d. NOTA

A

d. NOTA

77 ELEKTRONIKA URISED 2 AUTOMATED URINE SEDIMENT IS THE CORRECT ANSWER

58
Q

Fluorescent flow cytometry

a. Sysmex UF-1000 and Sysmex UN-2000
b. Beckman’s Coulter iq200 microscopy analyzer
c. both
d. NOTA

A

a. Sysmex UF-1000 and Sysmex UN-2000

59
Q

1.3 mL of centrifuged urine

a. Beckman’s Coulter iq200 microscopy analyzer
b. 77 Elektronika UriSed 2 Automated Urine Sediment Analyzer
c. both
d. NOTA

A

d. NOTA

BECKMAN SHOULD BE 1.3 mL UNCENTRIFUGED URINE

60
Q

Digital camera

a. Beckman’s Coulter iq200 microscopy analyzer
b. FUS-100 and FUS-200 Sediment Analyzers
c. both
d. NOTA

A

c. both

61
Q

Phase-contrast microscope

a. 77 Elektronika UriSed 2 Automated Urine Sediment Analyzer
b. 77 Elektronika UriSed 3Pro Automated Urine Sediment Analyzer
c. both
d. nOTA

A

b. 77 Elektronika UriSed 3Pro Automated Urine Sediment Analyzer

62
Q

Centrifuged urine

a. 77 Elektronika UriSed 2 Automated Urine Sediment Analyzer
b. 77 Elektronika UriSed 3Pro Automated Urine Sediment Analyzer
c. both
d. nOTA

A

c. both

63
Q

Uncentrifuged urine

a. Sysmex UF-1000i and Sysmex UN-2000 Automated Urinalysis System
b. Beckman Coulterʼs iQ200 Microscopy Analyzer
c. FUS-100 and FUS-200 Sediment Analyzers
d. ALL
e. NOTA

A

d. ALL

64
Q

Automated urine system for sediments/cells

How many particle images does 77 Elektronika UriSed 2 Automated Urine Sediment Analyzer capture and categorize?

77 Elektronika UriSed 2 Automated Urine Sediment Analyzer

A

15 particle images

using AIEM software

AUBF (45 decks)

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