F2: CSF (Part 5: Tests - TUBE 3)

Question 1

What test/section in the laboratory is done in tube 3?

Answer 1

Hematology

Question 2

What is the normal WBC count in CSF?

Answer 2

less than 5 cells per ul

Question 3

Done if CSF is visibly clear to detect a small amount of pleocytosis

Answer 3

Tyndall’s Test

Question 4

Tyndall’s Test

Tyndall’s test procedure

a. Hold the tube at 90°angle in direct sunlight
b. take a laser light and flash
c. ALL
d. NOTA

Answer 4

c. ALL

Question 5

Tyndall’s Test

Negative result in tyndall’s test

Answer 5

Clear

Question 6

Tyndall’s Test

Positive result in tyndall’s test

Answer 6

Very turbid

Question 7

Tyndall’s Test

T or F

When consistency is clear, dilution is used

Answer 7

F (If clear – don’t dilute)

Question 8

Tyndall’s Test

When sample is turbid, you dilute using?

Answer 8

WBC pipette

Question 9

Hematology – Cell Count

What (2) reagents are used to dilute CSF for cell counting?

Answer 9

• saline
• Turk Solution

Question 10

How many squares are counted for cell counting?

Answer 10

5 large squares

Question 11

Unit for cell count

Answer 11

cell/cmm

Question 12

What diluting fluid is used to remove RBC

Answer 12

3% acetic acid

Question 13

What should you do if the first CSF sample was not diluted?

Answer 13

Just rinse the pipette

Question 14

What should you do if the first CSF sample was diluted?

Answer 14

use same dilution (for the addition of acetic acid) as the first

Question 15

Equation in Hematology

Answer 15

𝑇𝑜𝑡𝑎𝑙 𝑐𝑒𝑙𝑙𝑠 𝑐𝑜𝑢𝑛𝑡𝑒𝑑 − 𝑊𝐵𝐶 𝑐𝑜𝑢𝑛𝑡𝑒𝑑 = 𝑅𝐵𝐶

Question 16

Cell count correction formula

Answer 16

• 𝑊𝐵𝐶 𝑎𝑑𝑑𝑒𝑑 = 𝑊𝐵𝐶 (𝑖𝑛 𝐵𝑙𝑜𝑜𝑑) × 𝑅𝐵𝐶 (𝑖𝑛 𝐶𝑆𝐹) 𝑅𝐵𝐶 ( 𝑖𝑛 𝐵𝑙𝑜𝑜𝑑)
• 𝑇𝑟𝑢𝑒𝑊𝐵𝐶= 𝑊𝐵𝐶𝑐𝑜𝑢𝑛𝑡𝑒𝑑 − 𝑊𝐵𝐶𝑎𝑑𝑑𝑒𝑑

Or Subtract 1 WBC per 700 RBC counted

Question 17

Hematology -Differential Count steps

Answer 17

1. Centrifuge 5-10 minutes
2. Transfer supernate to another tube
3. Resuspend
4. Smear
5. Dry 6.Stain

Question 18

How many cells are typically needed for a differential count?

Answer 18

100 cells

Question 19

How many cells might one drop of CSF contain?

Answer 19

As few as 3 cells

Question 20

What are the normal percentages of lymphocytes and monocytes in CSF?

Answer 20

70 % lymph, 30 % monocytes

Question 21

• increase number, or presence of any other significant cell ie neutrophil, eosinophil

Answer 21

Pleocytosis

Question 22

T or F

differential count is done in CSF if pleocytosis is observed

Answer 22

T

Question 22

How are very few cells in CSF reported?

Answer 22

cells per slide

Question 23

A differential count is not accurate in CSF with very few cells since there are (too many/ too few cells)

Answer 23

too few cells to perform differential

Question 24

• More accurate
• Add 0.1 ml of CSF and 1 drop of 30% albumin
• cells forces onto 6 mm monolayer, stain
• Uses only 1 objective
• NOt used anymore
• Used to concentrate any body fluid

Answer 24

Cytocentrifuge

Question 25

Cytocentrifuge

Cytocentrifuge

a. 0.1 ul of CSF
b. 1 drop of 30% albumin
c. ALL
d. NOTA

Answer 25

b. 1 drop of 30% albumin

a. 0.1 ul of CSF - should be 0.1 ml of CSF

Question 26

T or F

Cytocentrifuge uses 2 objectives since sample is not easily seen

Answer 26

F (only uses 1 slide since sample is easily seen)

Question 27

T or F

Differential Count may now be done using Body Fluid Analyser found in CM section

Answer 27

T

Question 28

CLINICAL CORRELATION

Determine character

Bacterial: Increase
Viral: Increase
TB: Increase
Fungal: Increase

Answer 28

WBC

Question 29

Determine character

Bacterial: Marked increase
Viral: Moderate increase
TB: Marked increase
Fungal: Moderate increase

Answer 29

Protein

Question 30

Determine character

Bacterial: Marked decrease
Viral: Normal
TB: Moderate decrease
Fungal: Normal or mild decrease

Answer 30

Sugar

Question 31

Determine character

Bacterial: >35 mg/dL
Viral: Normal
TB: >25 mg/dL
Fungal: >25 mg/dL

Answer 31

Lactate

Question 32

Determine character

Bacterial: Phadebact CRP
Viral:
TB: Pellicle
Fungal:

Answer 32

Other