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AUBF > M2: Microscopic Examination (Part 2: Sediment Examination Techniques) > Flashcards

M2: Microscopic Examination (Part 2: Sediment Examination Techniques) Flashcards

1
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

Consists of Crystal violet and Safranin O

A

Sternheimer Malbin Stain

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2
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

Action: Delineates structure and contrasting colors of the
nucleus and cytoplasm

A

Sternheimer Malbin Stain

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3
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

Function: Provides a more detailed visualization of the
internal structure of WBCs, epithelial cells and casts

A

Sternheimer Malbin Stain

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4
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

RBC color result for in neutral, acidic, and alkaline urine?

Sternheimer Malbin Stain

A

neutral: pink to purple
acid: pink
alkaline: purple

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5
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

WBC nuclei and cytoplasm color result

Sternheimer Malbin Stain

A

nuclei: purple
cytoplasm: purple granules

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6
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

Squamous nucleus and cytoplasm color result

Sternheimer Malbin Stain

A

Nucleus: orange-purple
Cytoplasm: light purple or blue

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7
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

Bacteria color result for motile and non-motile

Sternheimer Malbin Stain

A

motile: unstained
non-motile: purple

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8
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

Action: Enhances nuclear detail
Function: Differentiates WBCs and renal tubular epithelial
(RTE) cells

A

0.5% Toluidine Blue

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9
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

Action: Lyses RBCs and enhances nuclei of WBCs
Function: Distinguishes RBCs from WBCs, yeast, oil
droplets, and crystals

A

2% acetic acid

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10
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

Enhances nuclear detail/nuclei

a. Sternheimer Malbin Stain
b. 0.5% Toluidine Blue
c. 2% acetic acid
d. a and c
e. b and c
f. ALL
g. NOTA

A

e. b and c

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11
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

Action: Stain triglycerides and neutral fats orange-red (Do
not stain cholesterol)
Function: Identify free fat droplets and lipid-containing cells and casts

A

Lipid stains: Sudan III or Oil Red O

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12
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

T or F

To confirm presence of lipids triglycerides, neutral fats, and cholesterol) from other sediments such as free fat droplets and lipid-containing cells and casts in the urinary sediment, staining is only used

Lipid stains: Sudan III or Oil Red O

A

F (staining and polarization)

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13
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

T or F
Sudan III or Oil Red O stains cholesterol

Lipid stains: Sudan III or Oil Red O

A

F (does not)

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14
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

Action: Stains structures containing iron
Function: Identifies yellow-brown granules of hemosiderin in cells and casts.

A

Prussian Blue

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15
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

Prussian Blue result for presence of homosiderin

Prussian Blue

A

Blue

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16
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

Action: Methylene blue and eosin Y stains eosinophilic granules
Function: Identifies urinary eosinophils

A

Hansel Stain

17
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

Action: Differentiates gram-positive and gram-negative
bacteria
Function: Identifies bacterial casts

A

Gram stain

18
Q

SEDIMENT EXAMINATION TECHNIQUES: URINE SEDIMENT STAINS

To perform gram staining, what specimen is required?

A

dried, heat-fixed preparation of
the urine sediment

19
Q

If you see this card pakireview yung table sa trans thanks mwah

A

go na i swear

20
Q
  • the preparation of permanent slides using cytocentrifugation followed by staining with Papanicolaou stain
  • provides an additional method for
    detecting and monitoring renal disease.
A

Cytodiagnostic urine testing

21
Q

CYTODIAGNOSTIC URINE TESTING

what stain is used after cytocentrifugation?

A

Papanicolaou stain

22
Q

Frequently performed independently of routine urinalysis for detection of malignancies of the lower urinary tract.

A

Cytodiagnostic urine testing

23
Q

Provides more definitive information about renal tubular
changes associated with
* transplant rejection
* viral, fungal, parasitic infections
* cellular inclusions
* pathologic casts
* inflammatory condition

A

Cytodiagnostic urine testing

24
Q

What specimen is recommended for testing, which is performed by the cytology laboratory

A

voided first morning specimen

AUBF (45 decks)

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