F1: Semen Analysis (Part 2: Specimen Collection and Handling)

Question 1

SPECIMEN COLLECTION AND HANDLING

T or F

Collection of semen should be complete

Answer 1

T

Question 2

SPECIMEN COLLECTION AND HANDLING

Specimen is collected in?

Answer 2

container

Question 3

SPECIMEN COLLECTION AND HANDLING

First or Last portion of the ejaculate?

• Contains most of the
  sperm cells & prostatic
  fluid

Answer 3

First portion of the
ejaculate

Question 4

SPECIMEN COLLECTION AND HANDLING

First or Last portion of the ejaculate?

If this portion of
ejaculate is missing:
* Sperm count is decreased

Answer 4

First portion of the
ejaculate

Question 5

First or Last portion of the ejaculate?

If this portion of
ejaculate is missing:
* pH is falsely increased

Answer 5

First portion of the
ejaculate

Question 6

First or Last portion of the ejaculate?

If this portion of
ejaculate is missing:
* Specimen will not liquefy

Answer 6

First portion of the
ejaculate

Question 7

First or Last portion of the ejaculate?

Most of the fluid from
seminal vesicle

Answer 7

Last portion of the ejaculate

Question 8

First or Last portion of the ejaculate?

If this portion of
ejaculate is missing:
* Semen volume is decreased

Answer 8

Last portion of the
ejaculate

fluid volume hbshcbhs ok

Question 9

First or Last portion of the ejaculate?

If this portion of
ejaculate is missing:
* Sperm count is falsely increased

Answer 9

Last portion of the
ejaculate

Question 10

First or Last portion of the ejaculate?

If this portion of
ejaculate is missing:

pH is falsely decreased

Answer 10

Last portion of the
ejaculate

magiging acidic, kasi wala na alkalinity awww

Question 11

First or Last portion of the ejaculate?

If this portion of
ejaculate is missing:
* Specimen will not clot

Answer 11

Last portion of the
ejaculate

wala na nung clotting proteins ok?

Question 12

SPECIMEN COLLECTION AND HANDLING

Which of the following are not included in the requisition form?

• Patient’s name and birth date
• Period of sexual abstinence
• Completeness of the sample
• Easyness with collection
• Time of specimen collection and specimen receipt

Answer 12

Easyness with collection (SHOULD BE DIFFICULTIES with collection)

Question 13

4 collection methods of sperm could be?

Answer 13

1. Masturbation
2. Interrupted sexual interocourse
3. Condom
4. Aspiration from vaginal canal after intercourse

Question 14

4 collection methods of sperm

• Self-production
• Commonly used; best
• Patients can collect from the laboratory as they are provided with collection room

Answer 14

Masturbation

Question 15

4 collection methods of sperm

• termed as “Coitus interruptus”
• Not advisable

Answer 15

After interrupted sexual intercourse

Question 16

4 collection methods of sperm

• Should be free from spermicidal agents
• Non-lubricant containing polymeric silicone (silastic) or polyurethane condoms

Answer 16

Condom

Question 17

4 collection methods of sperm

T or F

Condom should have libricant or flavor to inhibit spermicidal agents

Answer 17

F (should have none of these things so that its spermicidal)

Question 18

4 collection methods of sperm

Aspiration sample should be collected after how many hours of intercourse

after stopping ha hindi ilang hours nag intercourse

Answer 18

2 – 6 hours (depends on the doctor)

Question 19

4 REQUIREMENTS PRIOR TO COLLECTION

Familiarize the 4 requirements prior to collection

Answer 19

• Empty bladder before ejaculation
• Sexual abstinenc (2-7 days)
• Fertility testing (2-3 samples)
• Warm sterile glass or plastic containers

Question 20

4 REQUIREMENTS PRIOR TO COLLECTION

T or F

Urine is toxic to sperm cells hence there should be empty bladder before ejaculation

Answer 20

T

Question 21

4 REQUIREMENTS PRIOR TO COLLECTION

For how many days is sexual abstinence before collection?

Answer 21

2 – 7 days

Question 22

4 REQUIREMENTS PRIOR TO COLLECTION

T or F

Sexual abstinence is done to get higher volume of sperm

Answer 22

T

Question 23

4 REQUIREMENTS PRIOR TO COLLECTION

T or F

Prolonged: low volume or low count of sperm

Short: high volume or high count of sperm

Answer 23

F (reverse)

Question 24

4 REQUIREMENTS PRIOR TO COLLECTION

T or F

Abstinence results in decreased motility of sperm

Answer 24

T

Question 25

4 REQUIREMENTS PRIOR TO COLLECTION

Fertility testing should have how many samples?

Answer 25

2-3 samples

Question 26

4 REQUIREMENTS PRIOR TO COLLECTION

Samples should not be less than (blank) or more than (blank) apart

Fertility testing

Answer 26

should not be less than 7 days or
more than 3 weeks apart

Question 27

4 REQUIREMENTS PRIOR TO COLLECTION

Significant result means there should be how many abnormal samples?

Answer 27

2 abnormal samples

Question 28

4 REQUIREMENTS PRIOR TO COLLECTION

If a plastic container is used, sample should be examined within?

Answer 28

within 1 hour

Plastic has inhibitory effect on motility after 60 minutes

Question 29

4 REQUIREMENTS PRIOR TO COLLECTION

After 1 hr, sample collected using plastic is inhibitory on?

Answer 29

motility of sperm

Question 30

REQUIREMENTS FOR TRANSPORT

Familiarize the 2 requirements for transport

Answer 30

• Kept at room or body temperature 37°C
• Delivered to the laboratory within 1 hour of collection

Question 31

REQUIREMENTS FOR TRANSPORT

Transport of specimen should be kept at what temperature?

dalawa

Answer 31

Room, Body temperature (37)

Question 32

SPECIMEN HANDLING

T or F

Specimen handling includes:
* Observing standard precautions
* Treating semen as potential reservoir for HIV and hepa virus
* Discarded as regular waste

Answer 32

F (all are correct except last)

should be: semen is discarded as biohazard waste

Question 33

BASIC EXAMINATION (WHO) includes what 3 parameters?

Answer 33

• Sperm concentration
• Motility
• Morphology percentage