M15: Diagnosis of Infectious Disease Flashcards
When does the colonisation by microorganisms on the body begin?
Human are usually germ-free in utero and microorganisms colonisation of the body begins at birth
Give some locations of normal microbiota on and in the human body (7)
- Nose
- Mouth
- Lungs
- Skin
- Stomach
- Gut
- Vagina/Uretra
“Normal microbiota and the host exist in symbiosis”
Define symbiosis
Long-term interaction between two or more different biological species
What are opportunistic pathogens?
- Only cause disease in compromised host
- Sometimes part of Normal Flora
Define pathogenicity
The ability of the microbe to cause disease (qualitative)
Define virulence
The quantitative ability of a microbe to cause disease
Name the 4 basic microorganisms that can cause infections in humans
- Bacteria
- Viruses
- Fungi
- Protozoans (parasites)
What is the role of the capsule in a bacteria?
Help to evade the immune response
What is the role of spores in bacteria?
Give resistance to physical and chemical agents
Comment on the cell wall of gram positive bacteria and what colour do they stain?
- Thick peptidoglycan with no outer membrane
- Purple
Comment on the cell wall of gram negative bacteria and what colour do they stain?
- Thinner peptidoglycan with outer membrane
- Pink
What are the 3 main shapes of bacteria?
- Cocci (round)
- Rod
- Curved
How are cocci arranged?
Cocci are spherical chains arranged in a cluster
How do you carry out a gram stain?
- Put colonies on a slide
- Apply crystal violet (purple dye)
- Apply iodine
- Alcohol wash for decolorisation
- Apply safarin (pink dye)
- Observe
How do you distinguish between streptococci and staphylococci?
- Catalase test
- Streptococci are catalase negative
- Staphylococci are catalase positive
[e in strep e in negative]