LIPASE (LAB)

Question 1

This substrate is cleaved by the pancreatic lipase

Answer 1

1,2-o-Dilauryl-rac-glycero-3-glutaric acid-(6’ methyl-resorufin) ester

Question 2

The colorimetric substrate 1,2-o-Dilauryl-rac-glycero-3-glutaric acid-(6’ methyl-resorufin) ester is cleaved by
pancreatic lipase and the resulting ____________________ is hydrolyzed under alkaline conditions to yield the _____________________.

Answer 2

dicarboxylic acid ester
chromophore
methylresorufin.

Question 3

What is the volume of SERUM, CALIBRATOR, and BLANK in the LIPASE experiment?

Answer 3

20 uL

Question 4

What is the volume of R1 in the LIPASE activity?

Answer 4

1000 uL

Question 5

What is the volume of the R2 in the LIPASE ACTIVITY?

Answer 5

250 uL

Question 6

What is the absorbance of lipase?

Answer 6

580nm

Question 7

After all the absorbance has been averaged, what would be the next step?

Answer 7

Subtract Black from Serum to get the Final serum value

and,

Subract Blank from the Calibrator value to get the final calibrator value.

Question 8

What is the formula of LIPASE?

Answer 8

Sample divided by the calibrator, multiplied to the calibrator value.

Question 9

What is the normal value of LIPASE?

Answer 9

≤60 U/L