CREATINE KINASE Flashcards
What is the reaction of CK?
Creatine + ATP <> Phosphocreatine + ADP
What is the major phosphorylated compound in muscle?
Phosphocreatine
When muscles contracts, what happen?
ATP is consumed to form ADP.
CK catalyzes the resphosphorylation of ADP to for ATP
In terms of tissue course of CK, enumerate where it it GREATEST and SMALLER QUANTITIES.
GREATEST:
Striated muscle and Heart tissue
SMALLER QUANTITIES:
Bladder, Placenta, GIT, Thyroid, Uterus, KIdney, Lung, Prostate, Spleen and Pancreas
How much protein CK activity is present is striated muscle and Heart tissue?
Striated muscle : 2,500
Heart tissue: 500
Which is devoid of CK activity?
Liver and RBC
What is the structure of CK?
A DIMER with 2 subunits
What is the MW of each subunit?
40, 000 Dalton’s
What are the 3 important Isoenzymes of the CK?
CK-BB (CK1)
CK-MB (CK2)
CK-MM (CK3)
The 3 important isoenzymes are mainly found in what part?
The cytosol
What are the forms of CK?
Type 1 and Oligomeric Mitochondrial CK.
What type of macromolecular form of CK has the Isoenzymes of CK forms complex with the Immunoglobulin?
Type 1
What type of Macromolecular form of CK is commonly found in adults with malignancy or liver conditions?
Oligomeric Mitochondrial conditions
CK1, CK2 and CK3 can be found in?
CK1
- Brain
- Prostate
- GUt
- Lung
- Bladder
- Uterus
- Placenta
- Thyroid
CK2
- Heart muscles
CK3
- Skeletal (predominates)
- Cardiac muscle
What do you call the 4th ISoenzyme?
CK-Mt (MItochodrial Isoenzyme)
To which does CK-Mt differ?
Immunologically and in electrophoretic mobility
CK-Mt constitutes up to ________ CK activity in the heart.
15%
This diagnostic significance of CK refers to a viral infection caused by entero virus and etc.
Viral myositis
This disease talks about an autoimmune disorder that happens in the muscle?
Polymyositis
Muscular dystrophy Duchenne type increases up to?
50x ULN
In this disease, the problem is the nerves.
Neurogenic muscle disease
What are the examples of Neurogenic Muscle Disease?
Myasthenia gravis
Multiple sclerosis
Poliomyelitis
Parkinsonism
Rhabdomyolisis
This NMD produce antibodies against nerves that control our muscles.
Myasthenia gravis
This disorder has the brain and the spinal cord conditions
Multiple sclerosis