AMYLASE (LAB) Flashcards
1
Q
The hydrolysis of this reaction is catalyzed by the Amylase.
A
2-chloro-4-nitrophenyl-α-D-maltotrioside
(CNPG3)
2
Q
The enzyme α-amylase (E.C. 3.2.1.1) catalyzes the hydrolysis of 2-chloro-4-nitrophenyl-α-D-maltotrioside
(CNPG3) to release __________________ and form __________________ (CNPG2)
A
2-chloro-4-nitrophenol
2-chloro-4-nitrophenyl-α-D-maltoside
3
Q
At what absorbance can Amylase be detected spectrophotometrically?
A
405 nm.
4
Q
What is the volume of R1?
A
1000
5
Q
IN THE AMYLASE ACTIVITY, IS SERUM ADDED BEFORE OR AFTER INCUBATION?
A
After incubation
6
Q
What is the value where the AMS change per minute is multiplied to?
A
3178
7
Q
What is the normal value of Serum/Plasma in Amylase?
A
<96
8
Q
What is the normal value of AMYLASE in terms of ukat/L?
A
<1.60 ukat/L
