ACID PHOSPHATASE Flashcards
What is the systematic name of ACP?
E.C. 3.1.3.2
ACP and ALT are both hydrolase. Therefore what do they catalyze?
Phosphate monoesters
TRUE OR FALSE:
The substrate of ACP will always have a “Phosphate”
TRUE
What is the optimum pH that ACP takes place?
<7 pH
ACP isoenzymes are divided into 2 groups based on?
Inhibition
This refers to the activity being stopped or inactivated.
Inhibition
What are the INHIBITORY CHEMICAL REAGENTS?
- 2% Formaldehyde
- Cupric Sulfate
- Tartrate
This is the commonly used inhibitory chemical reagents.
Tartrate
What are the 2 groups of Isoenzymes?
A) Tartrate Resistant ACPs
B) Inhibited by chemical reagents
What are the Isoenzymes that belongs to TRAP?
Bone & Red Blood Cell ACP
This is an isoenzyme that resists inhibition caused by reagents.
Tartrate Resistant ACPS
What are the Isoenzymes that are inhibited by chemical reagents (Tartrate)?
Prostatic ACP
Platelets, Granulocytes and Monocytes
All ACP activities are located in the _____________ of the cell.
Lysosome
This is an organ within the cytoplasm of the cell except for the RBC.
Lysosome
Why is ACP activity not found in RBC?
Because it has no lysosome. And all ACP activities are found within the lysosome.
What Isoenzymes belongs to bands 1, 2, 3, 4, and 5?
1 : Prostatic ACP
2 & 4 : Granulocytes
3 : Platelets, RBC’s and Monocytes
5 : Bone - osteoclasts
These Isoenzymes are inhibited by Tartrate.
Prostatic ACP and Granulocytes, Platelets and MONOCYTES
These are the major form in plasma
Platelets, RBCs and Monocytes
These Isoenzymes are resistant to Tartrate inhibition
Bone - osteoclasts and RBCs
What causes the ACP elevation?
Prostatic Isoenzyme and Bone Isoenzyme/TRAP
What is the most common cancer among men?
Prostate cancer