LESSON 2 (part2) Flashcards
This talks about the hydrogen Ion concentration
pH
This talks about the point which the concentration rate is greatest.
Optimum pH
At what pH does many enzymes shows maximum activity?
7.0 - 8.0
At what pH is Pepsin active?
1.5
At what pH is ALP active?
10.5
This binds the substrate to the active site by forming ionic bridges and orients the substrate so it is attached to the enzyme in the correct configuration.
Activators
What is the common activator?
Magnesium
This decreases the rate of enzyme reaction and counterpart of activators.
Inhibitors
Describe the ff:
A. Competitive Inhibition
B. Non-competitive Inhibition
C. Uncompetitive Inhibition
A. Competes with the substrate
B. Freely attached and will not compete to the substrate.
C. The inhibitor will bind to the E-S complex
Enumerate the type of Inhibition
A. Reversible INhibition
B. Irreversible Inhibition
Describe the following:
A. Reversible INhibition
B. Irreversible Inhibition
a. Inhibitors are possibly removed from the system. Therefore, the enzyme is fully restored.
B. Inhibitors covalently combined with the enzyme
What are the examples f iNhibitors?
- Excess substrate
- Product of Inhibition
- E-S complex does not break to yield products
- Chemical substances
This inhibitor causes COMPETITION between substrate and molecules for a single binding site.
Excess substrate
This maybe an inhibitor of the forward reaction.
Product of reaction
This is the loss of enzymatic activity.
Enzyme denaturation